Incubation of a crude yeast extract containing phosphofructokinase with proteinase A, proteinase B or carboxypeptidase Y gave the following results: Proteinase B and carboxypeptidase Y did not change the activity of phosphofructokinase during incubation. On the other hand, incubation with proteinase A resulted in a 40-100% activation; continued incubation, however, led to an inactivation of the enzyme. Addition of allosteric effectors did not change the activation or inactivation process. The activated phosphofructokinase was not changed with respect to pH optimum and ATP inhibition. Molecular weight determination of phosphofructokinase in crude extracts in the presence of inhibitors of proteinase A indicated a molecular weight of 700000. Without inhibitors of proteinase A, the molecular weight was determined to be 600 000, while after 40-100% activation by proteinase A, a molecular weight of 500 000 was obtained. The activity profile of proteinase A in density gradients indicated that this enzyme is bound to variety of cellular proteins.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1515/bchm2.1976.357.2.1771 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Experimental and computational approach on the corrosion inhibition properties of two newly pyrazole derivatives on carbon steel in acid medium.
Sci Rep
January 2025
LCEA Laboratory, Faculty of Sciences, Mohammed Premier University, Oujda, Morocco.
In the current investigation, the efficiency inhibition of two newly synthesized bi-pyrazole derivatives, namely 2,3-bis[(bis((1 H-pyrazol-1-yl) methyl) amino)] pyridine (Tetra-Pz-Ortho) and 1,4-bis[(bis((1 H-pyrazol-1-yl) methyl) amino)] benzene (Tetra-Pz-Para) for corrosion of carbon steel (C&S) in 1 M HCl medium was evaluated. A Comparative study of inhibitor effect of Tetra-Pz-Ortho and Tetra-Pz-Para was conducted first using weight loss method and EIS (Electrochemical Impedance Spectroscopy) and PDP (Potentiodynamic Polarisation) techniques. Tetra-Pz-Ortho and Tetra-Pz-Para had a maximum inhibition efficacy of 97.
View Article and Find Full Text PDFAccurate Dehydrogenation Enthalpies Dataset for Liquid Organic Hydrogen Carriers.
Sci Data
January 2025
Materials Science Division, Argonne National Laboratory, Lemont, IL, 60439, USA.
This contribution presents a comprehensive extension of the QM9 dataset (originally at 133 K molecules) with the calculation of G4MP2 enthalpies for 9,841 molecules, featuring up to nine heavy atoms. We present QM9-LOHC, a (de)hydrogenation dataset of 10,373 reactions, including a minimum of 5.5% weight hydrogen storage capacity in line with the Department of Energy standards for Liquid Organic Hydrogen Carriers (LOHC).
View Article and Find Full Text PDFScreening and Preparation of Nanobodies for SIGLEC-15 Detection.
Protein Expr Purif
January 2025
Department of Pharmacy, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China; National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital of Chinese Academy of Medical Sciences, Langfang Campus, Langfang, 065001, China. Electronic address:
As an important member of the Siglec family, SIGLEC-15 plays an important role in osteoclast differentiation, bone remodeling, and tumor immune evasion. In the tumor microenvironment, SIGLEC-15 functions independently of the B7-H1/PD-1 pathway. In this study, the SIGLEC-15 fusion protein (SIGLEC-15-Fc) was successfully expressed and purified using a eukaryotic expression system.
View Article and Find Full Text PDFA bioactive polysaccharide derived from Rosa laevigata fruits: Structural properties, antitumor efficacy, and potential mechanisms.
Int J Biol Macromol
January 2025
State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China. Electronic address:
A heteropolysaccharide, designated JYP70-1, was extracted and purified from the fruits of Rosa laevigata, exhibiting a molecular weight of 1.90 × 10 g/mol. Structural analysis revealed that JYP70-1 was composed of eleven sugar residues, including α-l-Araf-(1→, →3)-α-l-Araf-(1→, →5)-α-l-Araf-(1→, →3,5)-α-l-Araf-(1→, →2,5)-α-l-Araf-(1→, →4)-α-d-Galp-(1→, →6)-β-d-Galp-(1→, →6)-α-d-Glcp-(1→, α-d-Glcp-(1→, →2)-α-d-Manp-(1→, and →3,6)-β-d-Manp-(1→.
View Article and Find Full Text PDFPurification, structural characterization, and in vitro immunomodulatory activity of a low-molecular-weight polysaccharide from cultivated Chinese cordyceps.
Int J Biol Macromol
January 2025
Key Laboratory of State Administration of Traditional Chinese Medicine, Dongguan HEC Cordyceps R&D Co., Ltd., Dongguan, Guangdong 523850, China; College of Medical Imaging Laboratory and Rehabilitation, Xiangnan University, Chenzhou, Hunan 423000, China. Electronic address:
Cultivated Chinese cordyceps, an optimal substitute for the endangered wild resource, has recently been produced on a large scale. This work sought to explore the structural features and immunomodulatory activity of a novel low-molecular-weight polysaccharide (CSP1a, 15.7 kDa) isolated from cultivated Chinese cordyceps.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!