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Immature Skeletal Myotubes Are an Effective Source for Improving the Terminal Differentiation of Skeletal Muscle.
Cells
December 2024
Department of Physiology, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.
Injured or atrophied adult skeletal muscles are regenerated through terminal differentiation of satellite cells to form multinucleated muscle fibers. Transplantation of satellite cells or cultured myoblasts has been used to improve skeletal muscle regeneration. Some of the limitations observed result from the limited number of available satellite cells that can be harvested and the efficiency of fusion of cultured myoblasts with mature muscle fibers (i.
View Article and Find Full Text PDFMyofibers cultured in viscoelastic hydrogels reveal the effects of integrin-binding and mechanosensing on muscle satellite cells.
Acta Biomater
January 2025
Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder CO, 80303, USA; The BioFrontiers Institute, University of Colorado Boulder, Boulder, CO 80303, USA. Electronic address:
Quiescent skeletal muscle satellite cells (SCs) located on myofibers activate in response to muscle injury to regenerate muscle; however, identifying the role of specific matrix signals on SC behavior in vivo is difficult. Therefore, we developed a viscoelastic hydrogel with tunable properties to encapsulate myofibers while maintaining stem cell niche polarity and SC-myofiber interactions to investigate how matrix signals, including viscoelasticity and the integrin-binding ligand arginyl-glycyl-aspartic acid (RGD), influence SC behavior during muscle regeneration. Viscoelastic hydrogels support myofiber culture while preserving SC stemness for up to 72 hours post-encapsulation, minimizing myofiber hypercontraction and SC hyperproliferation compared to Matrigel.
View Article and Find Full Text PDFAttachment promoting compounds significantly enhance cell proliferation and purity of bovine satellite cells grown on microcarriers in the absence of serum.
Front Bioeng Biotechnol
November 2024
Mosa Meat BV, Maastricht, Netherlands.
Introduction: To bring cultivated beef to the market, a scalable system that can support growth of bovine satellite cells (bSCs) in a serum-free and preferably also animal-free medium is of utmost importance. The use of microcarriers (MCs) is, at the moment, one of the most promising technologies for scaling up. MCs offer a large surface to volume ratio, they can be used in scalable stirred tank bioreactors, where the culture conditions can be tightly controlled to meet the cells' requirements (temperature, pH, dissolved oxygen).
View Article and Find Full Text PDFExchange of free and capsule conjugated cyanine dyes between cells.
J Mater Chem B
December 2024
Vladimir Zelman Center for Neurobiology and Brain Rehabilitation, Skoltech, Bolshoy Boulevard 30., Moscow 121205, Russia.
Fluorescent dyes (especially photoconvertible cyanine dyes) are traditionally used as labels to study single-cell or cell-group interactions and migration. Nevertheless, their application has some disadvantages, such as cytotoxicity and dye transfer between cells during co-cultivation. The latter can lead to serious distortions in research results.
View Article and Find Full Text PDFMechanisms of Chimeric Cell Therapy in Duchenne Muscular Dystrophy.
Biomedicines
September 2024
Dystrogen Therapeutics Technology Polska sp. z o.o., 00-777 Warsaw, Poland.
Despite scientific efforts, there is no cure for Duchenne muscular dystrophy (DMD), a lethal, progressive, X-linked genetic disorder caused by mutations in the dystrophin gene. DMD leads to cardiac and skeletal muscle weakness, resulting in premature death due to cardio-pulmonary complications. We have developed Dystrophin Expressing Chimeric (DEC) cell therapy, DT-DEC01, by fusing human myoblasts from healthy donors and from DMD patients.
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