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Transgenic tomato strategies targeting whitefly eggs from apoplastic or ovary-directed proteins.
BMC Plant Biol
December 2024
Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.
Background: Transgenic plants expressing proteins that target the eggs of the ubiquitous plant pest Bemisia tabaci (whitefly) could be an effective insecticide strategy. Two approaches for protein delivery are assessed using the mCherry reporter gene in transgenic tomato plants, while accommodating autofluorescence in both the plant, phloem-feeding whitefly and pedicle-attached eggs.
Results: Both transgenic strategies were segregated to homozygous genotype using digital PCR.
The Effect of Biochar on Tomato () Cultivar Micro-Tom Grown under Continuous Light.
J Soil Sci Plant Nutr
November 2024
Department of Chemistry, Faculty of Science and Engineering, Swansea University, Swansea, Wales SA2 8PP UK.
Unlabelled: Continuous lighting (CL) has the potential to increase crop yield in greenhouse production. Tomato plants, however, when exposed to CL develop inter-vascular chlorosis, a leaf injury which causes a reduction in chlorophyll content and necrosis. The application of biochar can reduce physiological stress in plants, we examine if biochar also reduces necrosis in tomatoes when grown under CL.
View Article and Find Full Text PDFQuantification of pesticide dosage and determinants of excessive pesticide use in smallholder vegetable production systems in Tanzania.
Heliyon
December 2024
Tanzania Plant Health and Pesticides Authority (TPHPA), P.O. Box 3024, Arusha, Tanzania.
The use of pesticides for diseases and insect pest control has become a key component in smallholder vegetable production. This study therefore quantified the concentration of pesticide active ingredient per unit production land (kg a.i/ha), and drivers of increased pesticide use in smallholder vegetable production systems in Tanzania.
View Article and Find Full Text PDFDifferential impact of impaired steryl ester biosynthesis on the metabolome of tomato fruits and seeds.
Physiol Plant
December 2024
Plant Synthetic Biology and Metabolic Engineering Program, Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Cerdanyola, Barcelona, Spain.
Steryl esters (SE) are a storage pool of sterols that accumulates in cytoplasmic lipid droplets and helps to maintain plasma membrane sterol homeostasis throughout plant growth and development. Ester formation in plant SE is catalyzed by phospholipid:sterol acyltransferase (PSAT) and acyl-CoA:sterol acyltransferase (ASAT), which transfer long-chain fatty acid groups to free sterols from phospholipids and acyl-CoA, respectively. Comparative mass spectrometry-based metabolomic analysis between ripe fruits and seeds of a tomato (Solanum lycopersicum cv Micro-Tom) mutant lacking functional PSAT and ASAT enzymes (slasat1xslpsat1) shows that disruption of SE biosynthesis has a differential impact on the metabolome of these organs, including changes in the composition of free and glycosylated sterols.
View Article and Find Full Text PDFParasitic Contamination and Its Associated Factors in Fruits and Vegetables Collected From Ethiopia's Local Markets: A Systematic Review and Meta-Analysis.
Environ Health Insights
December 2024
Department of Biology, College of Natural and Computational Science, Mizan-Tepi University, Tepi, Ethiopia.
Background: Vegetables and fruits are vital components of a healthy diet; however, consuming unclean, uncooked, or improperly prepared products can lead to parasitic infections. Contamination often occurs during both the pre-harvest and post-harvest phases. Thus, the aim of this study is to provide evidence-based scientific information about the level of parasitic contamination and risk factors in Ethiopian fruits and vegetables sold in local markets.
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