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Involvement of autophagy in germ cells in an experimental model of varicocele in rats before and after varicocelectomy.
Pediatr Surg Int
December 2024
Unit of Pediatric Surgery, Department of Human Pathology of Adult and Childhood "Gaetano Barresi", University of Messina, 98121, Messina, Italy.
Introduction: Autophagy, a catabolic process enabling cellular organelles and proteins' reuse for energy, has been observed in varicocele models, but the effect of surgical treatment on this process remains unknown. This study aims to assess autophagy in varicocele models undergoing surgical correction.
Materials And Methods: Twenty-one adolescent male rats were induced with varicocele and divided into three groups: sham, varicocele, and varicocele with varicocelectomy.
Protocol for identifying sound-activated neurons in the inferior colliculus by cFos immunostaining.
STAR Protoc
December 2024
Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh EH8 9XD, UK; Simons Initiative for the Developing Brain, Patrick Wild Centre, University of Edinburgh, Edinburgh, UK. Electronic address:
cfos is an immediate early gene commonly used to identify neuronal activation. After loud sound stimulation, neurons in the inferior colliculus are activated and cFos is expressed in the nucleus. Here, we present a protocol for quantifying neuronal activity in response to auditory stimulation using cFos immunostaining in the mouse inferior colliculus.
View Article and Find Full Text PDFImmunohistochemical Characterization of Spermatogenesis in the Ascidian .
Cells
November 2024
Shimoda Marine Research Center, University of Tsukuba, 5-10-1, Shimoda 415-0025, Shizuoka, Japan.
Article Synopsis
- The study analyzes spermatogenic cells in the testis of a marine invertebrate, identifying their unique non-cystic growth pattern with various follicle sizes.
- The researchers classified the spermatogenic cells into distinct round and elongated stages, using specific antibodies to recognize key markers throughout the process.
- Findings suggest the presence of Sertoli cell-like characteristics in free cells outside the follicles and offer insights for future comparative studies on spermatogenesis.
Yellow grub diseases on two seasonal killifish (Cyprinodontiformes, Rivulidae): a histopathological study.
Parasitol Res
November 2024
Laboratorio de Histología y Embriología Descriptiva, Experimental y Comparada (LHYEDEC), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata (UNLP), La Plata, Buenos Aires, Argentina.
Article Synopsis
- Yellow grub disease, caused by the parasite Clinostomum spp., affects the muscles and organs of freshwater fish, particularly killifishes in the Neotropical region, which are already vulnerable due to their short lifespan.
- * The study investigates the inflammatory damage caused by this parasite in various organs of two killifish species, revealing severe reactions especially in the pancreas, ovaries, and testes.
- * The findings suggest that this parasitic infection could lead to low fertility or sterility in seasonal killifishes, greatly affecting their ability to reproduce before their habitats dry up.
Efficacy of ozone versus mesenchymal stem cell-derived microvesicles in ameliorating testicular changes after hypothyroidism in adult albino rats: a histological and immunohistochemical study.
Ultrastruct Pathol
November 2024
Medical Histology and Cell Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Article Synopsis
- This study aimed to investigate the effects of induced hypothyroidism on testicular health in adult rats, specifically looking at histological and biochemical changes.
- It compared the therapeutic effects of two treatments, ozone injections and mesenchymal stem cell-microvesicles (MSCs-MVs), on these testicular alterations.
- Results showed that while both treatments improved testicular conditions, MSCs-MVs were more effective in restoring spermatogenesis compared to ozone.
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