AI Article Synopsis
- An enhanced electrophoretic molecular karyotype of Aspergillus nidulans ATCC 28901 was achieved, enabling the separation of seven chromosomal bands and better resolution of chromosomes III and VI.
- The penicillin biosynthesis genes (pcbAB, pcbC, and penDE) are found clustered on chromosome VI, while the argB gene is positioned on chromosome III.
- Some other A. nidulans strains have a modified chromosome III that partially overlaps with chromosome VI, allowing clear mapping of the penicillin gene cluster on chromosome VI of strain ATCC 28901.
Article Abstract
An improved electrophoretic molecular karyotype of Aspergillus nidulans ATCC 28901 has been obtained by contour-clamped electric field gel electrophoresis, which separates seven chromosomal bands and allows resolution of chromosomes III and VI. The three genes of the penicillin biosynthetic pathway, pcbAB, pcbC, and penDE, encoding alpha-aminoadipyl-cysteinyl-valine synthetase, isopenicillin N synthase, and isopenicillin N acyltransferase, respectively, are clustered together on a chromosome of 3.0 Mg, corresponding to linkage group VI, whereas the argB gene was located on a chromosome of 3.4 Mb, corresponding to linkage group III. Three other strains of A. nidulans contained a modified chromosome III of about 3.1 Mb that overlaps with chromosome VI, forming a doublet. Resolution of chromosomes III and VI in strain ATCC 28901 allowed unequivocal mapping of the penicillin gene cluster on chromosome VI of A. nidulans.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC207390 | PMC |
| http://dx.doi.org/10.1128/jb.174.21.7063-7067.1992 | DOI Listing |
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