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The amount of colloidal calcium phosphate (CCP) complex associated with caseins (insoluble [INSOL] Ca) determines the body, texture, flavor, and breakdown of cheese constituents during aging. The continuous pH decline during cheesemaking because of lactic acid fermentation results in solubilization of INSOL Ca. Measuring INSOL Ca in such a dynamic and wide range pH system (6.

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Introduction: This study utilized a injectable curcumin (Cur)-infused calcium phosphate silicate cement (CPSC) for addressing defects caused by bone cancer, and evaluated its promoting bone regeneration and exerting cytotoxic effects on osteosarcoma cells.

Methods: The material's physicochemical properties, biocompatibility with osteoblasts, and cytotoxicity toward osteosarcoma cells were rigorously analyzed.

Results: The findings demonstrate that CPSC-Cur signicantly prolongs the setting time, which can be optimized by adding silanized cellulose nanober (CNF-SH) to achieve a balance between workability and mechanical strength.

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Bacterial activation level determines Cd(II) immobilization efficiency by calcium-phosphate minerals in soil.

J Hazard Mater

January 2025

National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address:

Soil mineral properties significantly influence the mobility of Cd(II) within the soil matrix. However, the limited understanding of how microbial metabolism affects mineral structure at the microscale poses challenges for in situ remediation. Here, we designed a model calcium-phosphate system in a urea-rich environment to explore the impact of different microbial activation levels on Cd(II) fixation at mineral interfaces.

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Primary Neuronal Culture and Transient Transfection.

Bio Protoc

January 2025

Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, Taiwan.

Primary neuronal culture and transient transfection offer a pair of crucial tools for neuroscience research, providing a controlled environment to study the behavior, function, and interactions of neurons in vitro. These cultures can be used to investigate fundamental aspects of neuronal development and plasticity, as well as disease mechanisms. There are numerous methods of transient transfection, such as electroporation, calcium phosphate precipitation, or cationic lipid transfection.

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Reverse Hill-Sachs lesions (RHSL) are common complications associated with posterior shoulder dislocations and represent a significant challenge for preserving joint stability and function. If untreated, these compression fractures of the anteromedial humeral head can compromise the integrity of the joint, predisposing patients to recurrent instability and arthropathy. While various treatment modalities exist, achieving an anatomic reduction of the defect while preserving the articular cartilage remains a desirable outcome, particularly in acute settings.

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