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High-resolution mapping of cell cycle dynamics during steady-state T cell development and regeneration in vivo.
Cell Rep
January 2025
Molecular Immunology, Justus-Liebig-University Giessen, 35392 Giessen, Germany. Electronic address:
Control of cell proliferation is critical for the lymphocyte life cycle. However, little is known about how stage-specific alterations in cell cycle behavior drive proliferation dynamics during T cell development. Here, we employed in vivo dual-nucleoside pulse labeling combined with the determination of DNA replication over time as well as fluorescent ubiquitination-based cell cycle indicator mice to establish a quantitative high-resolution map of cell cycle kinetics of thymocytes.
View Article and Find Full Text PDFStudy of the RNA splicing kinetics via in vivo 5-EU labeling.
RNA
September 2024
Center of Life Sciences, Skolkovo Institute of Science and Technology, Skolkovo 121205, Russia
Splicing is an important step of gene expression in all eukaryotes. Splice sites might be used with different efficiency, giving rise to alternative splicing products. At the same time, splice sites might be used at a variable rate.
View Article and Find Full Text PDFIn utero pulse injection of isotopic amino acids quantifies protein turnover rates during murine fetal development.
Cell Rep Methods
February 2024
Department of Biochemistry & Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis, St. Louis, MO 63110, USA. Electronic address:
Protein translational control is critical for ensuring that the fetus develops correctly and that necessary organs and tissues are formed and functional. We developed an in utero method to quantify tissue-specific protein dynamics by monitoring amino acid incorporation into the proteome after pulse injection. Fetuses of pregnant mice were injected with isotopically labeled lysine and arginine via the vitelline vein at various embyonic days, and organs and tissues were harvested.
View Article and Find Full Text PDFpulse injection of isotopic amino acids quantifies protein turnover rates during murine fetal development.
bioRxiv
May 2023
Department of Biochemistry & Biophysics, University of Pennsylvania, Philadelphia, PA 19104.
Protein translational control is highly regulated step in the gene expression program during mammalian development that is critical for ensuring that the fetus develops correctly and that all of the necessary organs and tissues are formed and functional. Defects in protein expression during fetal development can lead to severe developmental abnormalities or premature death. Currently, quantitative techniques to monitor protein synthesis rates in a developing fetus () are limited.
View Article and Find Full Text PDFEfficiency of Protein Renewal Is Limited by Feed Intake and Not by Protein Lifetime in Aging .
J Proteome Res
November 2022
Department of Chemistry, National University of Singapore (NUS), Singapore 117543, Singapore.
Protein turnover maintains the proteome's functional integrity. Here, protein turnover efficiency over time in wild-type was assessed using inverse [N]-pulse labeling up to 7 days after the egg-laying phase at 20 °C. Isotopic analysis of some abundant proteins was executed favoring data quality over quantity for mathematical modeling.
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