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Efficient secretory expression, purification, and characterization of lipase in Pseudomonas aeruginosa M18, with multifunctional applications in diagnostics.
World J Microbiol Biotechnol
January 2025
Zybio Inc, Chongqing, 400082, China.
Lipase (EC 3.1.1.
View Article and Find Full Text PDFProteomic and peptidomic profiling of spirulina-fortified probiotic powder formulations during in vitro digestion.
Int J Biol Macromol
January 2025
Environmenal Research and Innovation (ERIN) department, Luxembourg Institute of Science and Technology (LIST), 5 avenue des Hauts Fourneaux, L4362 Esch-sur-Alzette, Luxembourg. Electronic address:
This study reports on the influence of lactic acid fermentation on the proteomic and peptidomic profiles of spirulina protein isolate (SPI)-fortified, freeze-dried powders containing living Lacticaseibacillus rhamnosus GG (LGG) cells during in vitro digestion. For comparison, powders fortified with whey protein isolate (WPI) and pea protein isolate (PPI) were also evaluated. Prior to freeze-drying, the powder precursors were either non-treated or fermented.
View Article and Find Full Text PDFNon-reactive biochar and Bacillus pumilus RSB17-based healing powder: A sustainable solution for enhanced bacterial viability in self-healing mortar.
Sci Total Environ
January 2025
Department of Civil Engineering, National Institute of Technology Karnataka, Surathkal 575025, India.
Existing mortar uses self-healing powders that are based on mineral admixtures, whose reactive nature negatively impacts bacterial viability and diminishes their effectiveness over time. This study aims to develop non-reactive, sustainable biochar-based healing powders with extended bacterial viability to serve as self-healing admixture in bio-mortar. Biochar from coconut husk, coconut shell, and coconut leaf petiole was evaluated for compatibility with Bacillus pumilus RSB17, emphasizing bacterial growth and calcium carbonate precipitation.
View Article and Find Full Text PDFOptimizing lipopeptide bioactivity: The impact of non-ionic surfactant dressing.
J Pharm Anal
December 2024
MTA-HUN-REN TTK Lendület "Momentum" Peptide-Based Vaccines Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Budapest, H-1117, Hungary.
The aim of the research is to increase the applicability of lipopeptides as drugs. To this end, non-ionic triblock copolymers, namely poloxamers, were applied. The physico-chemical properties of poloxamers vary depending on the length of the blocks.
View Article and Find Full Text PDFAugmented silver sulfadiazine nanostructured lipid carriers impregnated collagen sponge for promoting burn wound healing.
Int J Biol Macromol
January 2025
Department of Pharmaceutics, Faculty of Pharmacy, University of Sadat City, P.O. Box 32897, Menoufia, Egypt; Nanomedicine Laboratory, Faculty of Pharmacy, University of Sadat City, P.O. Box 32897, Sadat City, Egypt. Electronic address:
Silver sulfadiazine (SSD) is a widely used antibacterial agent for burn wound treatment owing to its capability in re-epithelialization and wound healing. However, due to its low solubility, the need for an effective drug delivery system is mandatory. This study aimed to optimize SSD nanostructured lipid-based carriers (NLCs), incorporated in a collagen sponge form as an innovative topical dosage form for effective burn wound treatment.
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