The O-specific polysaccharide was obtained by mild degradation of the Salmonella arizonae O61 lipopolysaccharide with acid. It contained 2-acetamido-2-deoxy-D-glucose, 2-acetamidino-2,6-dideoxy-L-galactose (FucAm), and 7-acetamido-3,5,7,9-tetradeoxy-5-[(R)-3-hydroxybutyramido]-D- glycero-L-galacto-nonulosonic acid (Sug). On the basis of partial acid hydrolysis with 0.1 M HCl, solvolysis with anhydrous HF in methanol, and 1H- and 13C-NMR analysis (including 1H/13C inversely correlated spectroscopy for localisation of N-acyl substituents), it was concluded that the O-specific polysaccharide had the following structure. ----3)-alpha-L-FucAm-(1----3)-alpha-D-GlcNAc-(1----8)-beta-Sug+ ++-(2---- The O-antigen of S. arizonae O61 is structurally related to that of Pseudomonas aeruginosa O12, thus explaining the known serological cross-reactivity between these micro-organisms.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0008-6215(92)84002-a | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Salmonella Typhimurium derived OMV nanoparticle displaying mixed heterologous O-antigens confers immunogenicity and protection against STEC infections in mice.
Microb Cell Fact
January 2025
College of Veterinary Medicine, Southwest University, Tiansheng Road NO.2, Chongqing, China.
Shiga toxin-producing Escherichia coli (STEC) is one of the major pathogens responsible for severe foodborne infections, and the common serotypes include E. coli O157, O26, O45, O103, O111, O121, and O145. Vaccination has the potential to prevent STEC infections, but no licensed vaccines are available to provide protection against multiple STEC infections.
View Article and Find Full Text PDFStructural and Serological Characterization of Yet Another New O Antigen, O86, in Clinical Strains.
Int J Mol Sci
December 2024
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia.
Bacteria from the genus are facultative human pathogens, primarily attacking the urinary tract and wounds. A total of 85 O serogroups have been identified so far among these bacilli. Bprz 86 was isolated from the fistula of a patient in Łódź, Poland.
View Article and Find Full Text PDFSome Human Anti-Glycan Antibodies Lack the Ability to Activate the Complement System.
Antibodies (Basel)
December 2024
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Science, 117991 Moscow, Russia.
Naturally occurring human antibodies against glycans recognize and quickly eliminate infectious bacteria, viruses and aberrantly glycosylated neoplastic malignant cells, and they often initiate processes that involve the complement system. Using a printed glycan array (PGA) containing 605 glycoligands (oligo- and polysaccharides, glycopeptides), we examined which of the glycan-binding antibodies are able to activate the complement system. Using this PGA, the specificities of antibodies of the IgM and IgG classes were determined in the blood serum of healthy donors (suggested as mostly natural), and, then, using the same array, it was determined which types of the bound immunoglobulins were also showing C3 deposition.
View Article and Find Full Text PDFPhage-resistance alters Lipid A reactogenicity: a new strategy for LPS-based conjugate vaccines against Rissen.
Front Immunol
December 2024
Department of Agricultural Sciences, University of Naples Federico II, Portici, Italy.
serovar Rissen ( Rissen) is an emerging causative agent of foodborne diseases. The current emergence of antibiotic resistance makes necessary alternative therapeutic strategies. In this study, we investigated the potential of a phage-resistant strain of Rissen (R) as a tool for developing an effective lipopolysaccharide (LPS)-based vaccine.
View Article and Find Full Text PDFImmunological studies of Burkholderia multivorans O-antigen oligosaccharide-rsScpA193 conjugates as potential candidates for vaccine development.
Int J Biol Macromol
December 2024
National Glycoengineering Research Center and NMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-based Medicine, Shandong University, 72 Binhai Road, Qingdao 266237, China. Electronic address:
The Burkholderia cepacia complex (Bcc) is a group of phenotypically similar but genotypically diverse Gram-negative bacteria that pose a significant threat to public health worldwide. Due to the absence of effective therapies, the development of an effective vaccine against Bcc infection is urgently needed. Lipopolysaccharide (LPS) O-antigens of B.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!