Earlier we have cloned cDNA coding for a polypeptide that reacts with monoclonal antibodies specific for some cytoskeleton structures. This gene is homologous to the laminin receptor 67 KD. However, cDNA suffices only for a polypeptide of 32 Da, far smaller than the 67 rDa laminin receptor. We have constructed a vector that produces the fusion protein LBP-TrpE in the bacterial strain CAG-456. Our studies show that hybrid protein LBP-TrpE is able to interact with laminin. This result was confirmed by using following methods: immunoblotting, "ELISA" and affinity chromatography on laminin.
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