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Improving Lentiviral Transduction of Human Adipose-Derived Mesenchymal Stem Cells.
Hum Gene Ther
December 2022
Department of Orthopaedic Surgery, Keck School of Medicine of the University of Southern California, Los Angeles, California, USA.
Lentiviral transduction of human mesenchymal stem cells (MSCs) induces long-term transgene expression and holds great promise for multiple gene therapy applications. Polybrene is the most commonly used reagent to improve viral gene transfer efficiency in laboratory research; however, it is not approved for human use and has also been shown to impair MSC proliferation and differentiation. Therefore, there is a need for optimized transduction protocols that can also be adapted to clinical settings.
View Article and Find Full Text PDFCharge is an important determinant of hemodynamic and adverse cardiovascular effects of cationic drugs.
Pharmacol Res
December 2015
Cardiovascular Division, King's College London, Rayne Institute, St Thomas' Hospital, London SE17EH, UK.
Cationic compounds are diverse and atypical therapeutic substances. In the present study we examined whether a prototypical class effect of cationic drugs in the cardiovascular system exists and whether this might be predictable on the basis of chemistry. The dose-dependent effects of cationic compounds of varying molecular weights and charge were examined on the blood pressure (BP), heart rate (HR) and the ECG in anesthetized rats.
View Article and Find Full Text PDFPlatelet factor 4 impairs the anticoagulant activity of activated protein C.
J Biol Chem
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Haemostasis Research Group, Institute of Molecular Medicine, Trinity College Dublin, St James' Hospital, Dublin 8, Ireland.
Platelet factor 4 (PF4) is an abundant platelet alpha-granule chemokine released following platelet activation. PF4 interacts with thrombomodulin and the gamma-carboxyglutamic acid (Gla) domain of protein C, thereby enhancing activated protein C (APC) generation by the thrombin-thrombomodulin complex. However, the protein C Gla domain not only mediates protein C activation in vivo, but also plays a critical role in modulating the diverse functional properties of APC once generated.
View Article and Find Full Text PDFUtility of cell-permeable peptides for enhancement of virus-mediated gene transfer to human tumor cells.
Biotechniques
May 2006
A.I. Virtanen Institute, University of Kuopio, Kuopio, Finland.
Small increases in pH enhance retroviral vector transduction efficiency of NIH-3T3 cells.
Biotechnol Prog
October 2003
Department of Chemical Engineering, Northwestern University, Evanston, Illinois 60208-3120, USA.
Increases in pH between 7.1 and 7.7 increase the efficiency of polybrene (Pb)- and protamine sulfate (PS)-aided retroviral transduction of NIH-3T3 cells in a serum-lot-dependent manner.
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