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c-di-GMP phosphodiesterase ProE interacts with quorum sensing protein PqsE to promote pyocyanin production in .
mSphere
January 2025
School of Medicine, Southern University of Science and Technology, Shenzhen, China.
The universal bacterial second messenger bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) plays critical roles in regulating a variety of bacterial functions such as biofilm formation and virulence. The metabolism of c-di-GMP is inversely controlled by diguanylate cyclases (DGCs) and phosphodiesterases (PDEs). Recently, increasing studies suggested that the protein-protein interactions between DGCs/PDEs and their partners appear to be a common way to achieve specific regulation.
View Article and Find Full Text PDFIsolation and Characterization of Lytic Phages Infecting Clinical from Tunisia.
Antibiotics (Basel)
December 2024
Biochemistry and Biotechnology Laboratory LR01ES05, Faculty of Sciences of Tunis, University of Tunis El Manar, El Manar II, Tunis 2092, Tunisia.
: is an opportunistic pathogen that causes a wide range of infections worldwide. The emergence and spread of multidrug-resistant clones requires the implementation of novel therapeutics, and phages are a promising approach. : In this study, two phages, KpTDp1 and KpTDp2, were isolated from wastewater samples in Tunisia.
View Article and Find Full Text PDFDevelopment of colorimetric and fluorescent closed tube LAMP assay using simplified extraction for diagnosis of Meloidogyne enterolobii in root tissues.
Sci Rep
January 2025
Department of Parasitology, Phramongkutklao College of Medicine, Bangkok, 10400, Thailand.
Meloidogyne enterolobii, a guava root-knot nematode, is a highly virulent pest in tropical and subtropical regions causing galls or knots in roots of diverse plant species posing a serious threat to agriculture. Managing this nematode is challenging due to limitations in conventional identification based on isolation and microscopic classification requiring expertise and time. A colorimetric and fluorescent LAMP assay using simplified extraction method targeting rDNA-ITS region was developed to detect M.
View Article and Find Full Text PDFEfficacy of Vaccination with the DIVENCE Vaccine Against Bovine Viral Diarrhea Virus Types 1 and 2 in Terms of Fetal Protection.
Vet Med (Auckl)
December 2024
Hipra Scientific S.L.U., R&D Department, Amer, Spain.
Purpose: To demonstrate the efficacy of DIVENCE, a vaccine against BVDV types 1 and 2 (BVDV-1 and BVDV-2) transplacental infection, following a booster regimen in heifers.
Materials And Methods: Calves of two-to-three months of age were given two intramuscular doses three weeks apart and a booster vaccine six months later. Efficacy was evaluated by means of a challenge with virulent BVDV-1 or BVDV-2 administered via the intranasal route at 85 days of gestation.
Isolation and phylogenetic analysis of camel contagious ecthyma virus in Morocco.
Microb Pathog
January 2025
Research and Development, MCI Santé Animale, Lot. 157, Z. I., Sud-Ouest (ERAC) B.P: 278, Mohammedia, 28810, Morocco. Electronic address:
Article Synopsis
- Camel contagious ecthyma is a viral skin disease that significantly impacts camels and leads to economic losses; this study focuses on identifying and analyzing the virus in Morocco, where it was found in 4 out of 15 affected camels.
- The research involved isolating the virus from skin crust samples and conducting various tests including PCR, which identified genetic similarities to the Ethiopian strain and allowed classification into two genetic clades: Asian and African lineages.
- This study marks the first detection of the virus in North Africa and highlights the virus's opportunistic infection tendencies linked to stress in the animals, suggesting the need for further research to assess its behavior and help in vaccine development.
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