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Structural insights and antimicrobial synergy of a proto-galectin from the marine sponge Aiolochroia crassa.

Comp Biochem Physiol B Biochem Mol Biol

January 2025

Laboratório de Biotecnologia Marinha - BioMar-Lab, Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Campus do Pici s/n, bloco 871, Fortaleza, CE 60440-970, Brazil. Electronic address:

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Native mucus is heterogeneous, displays high inter-individual variation and is prone to changes during harvesting and storage. To overcome the lack of reproducibility and availability of native mucus, commercially available purified mucins, porcine gastric mucin (PGM) and mucin from bovine submaxillary gland (BSM), have been widely used. However, the question is to which extent the choice of mucin matters in studies of their interaction with polymers as their composition, structure and hence physicochemical properties differ.

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J Proteome Res

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Faculty of Pharmacy, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Higashi, Osaka 577-8502, Japan.

Minor acidic glycans, such as sulfated and phosphorylated glycans, constitute only a small fraction of biological glycome, making their analysis a considerable challenge. In this study, we developed a technique to analyze minor acidic -glycans in biological samples. First, efficient reaction conditions for the release of -glycans from the proteins were determined.

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