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PC12 Cell Line: Cell Types, Coating of Culture Vessels, Differentiation and Other Culture Conditions.
Cells
April 2020
Department of Basic Medical Sciences, Wroclaw Medical University, 50-556 Wroclaw, Poland.
The PC12 cell line is one of the most commonly used in neuroscience research, including studies on neurotoxicity, neuroprotection, neurosecretion, neuroinflammation, and synaptogenesis. Two types of this line are available in the ATCC collection: traditional PC12 cells grown in suspension and well-attached adherent phenotype. PC12 cells grown in suspension tend to aggregate and adhere poorly to non-coated surfaces.
View Article and Find Full Text PDFElectrodeposited Gold on Carbon-Fiber Microelectrodes for Enhancing Amperometric Detection of Dopamine Release from Pheochromocytoma Cells.
Anal Chem
August 2018
Department of Chemistry , University of Washington, Seattle , Washington 98195-1700 , United States.
Exocytosis is an ultrafast cellular process which facilitates neuron-neuron communication in the brain. Microelectrode electrochemistry has been an essential tool for measuring fast exocytosis events with high temporal resolution and high sensitivity. Due to carbon fiber's irreproducible and inhomogeneous surface conditions, however, it is often desirable to develop simple and reproducible modification schemes to enhance a microelectrode's analytical performance for single-cell analysis.
View Article and Find Full Text PDFIn vivo processing and release into the circulation of GFP fusion protein in arginine vasopressin enhanced GFP transgenic rats: response to osmotic stimulation.
FEBS J
July 2015
Ushimado Marine Institute, Graduate School of Natural Science and Technology, Okayama University, Japan.
Arginine vasopressin (AVP) is a neurohypophysial hormone synthesized as a part of a prepropeptide precursor containing the signal peptide, AVP hormone, AVP-associated neurophysin II and copeptin in the hypothalamic neurosecretory neurons. A transgenic (Tg) rat line expressing the AVP-eGFP fusion gene has been generated. To establish the AVP-eGFP Tg rat as a unique model for an analysis of AVP dynamics in vivo, we first examined the in vivo molecular dynamics of the AVP-eGFP fusion gene, and then the release of GFP in response to physiological stimuli.
View Article and Find Full Text PDFDevelopment, evolution and pathology of neocortical subplate neurons.
Nat Rev Neurosci
March 2015
Department of Physiology, Anatomy and Genetics, Le Gros Clark Building, University of Oxford, South Parks Road, Oxford OX1 3QX, UK.
Subplate neurons have an essential role in cortical circuit formation. They are among the earliest formed neurons of the cerebral cortex, are located at the junction of white and grey matter, and are necessary for correct thalamocortical axon ingrowth. Recent transcriptomic studies have provided opportunities for monitoring and modulating selected subpopulations of these cells.
View Article and Find Full Text PDFStructure and expression of a shrimp prohormone convertase 2.
Gen Comp Endocrinol
September 2012
Shrimp-Virus Interaction Laboratory (ASVI), National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathumthani 12120, Thailand.
Although many crustacean neuroendocrine hormones have been reported, the enzymes responsible for post-translational modification of neuroendocrine hormones have rarely been characterized. A prohormone convertase 2 (PC2)-like enzyme has been isolated from the optic lobe of the giant tiger shrimp, Penaeus monodon and referred as PmPC2. The full length cDNA sequence of PmPC2 has been identified and found to resemble evolutionarily conserved PC2 enzymes of vertebrates and invertebrates.
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