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First evidence of previously undescribed trichomonad species in the intestine of pigs?
Vet Parasitol
April 2012
Institute of Pathology and Forensic Veterinary Medicine, Department of Pathobiology, University of Veterinary Medicine, Veterinärplatz 1, A-1210 Vienna, Austria.
Article Synopsis
- Three different trichomonad parasites, including Tritrichomonas foetus, were found in pigs, with a previous study indicating 47% of Trichomonas-positive samples contained species other than T. foetus.
- Gene sequencing of the 18S rRNA gene revealed unique sequences in three pigs, showing moderate similarity to existing data, with sequences from pigs 1 and 3 being 92% similar to Hypotrichomonas acosta.
- Phylogenetic analysis suggests the existence of at least two new trichomonad species in pig intestines, indicating a diverse microbial presence.
Design and validation of an oligonucleotide probe for the detection of protozoa from the order Trichomonadida using chromogenic in situ hybridization.
Vet Parasitol
July 2010
Institute of Pathology and Forensic Veterinary Medicine, Department of Pathobiology, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria.
Infections with protozoal parasites of the order Trichomonadida are often observed in veterinary medicine. Based on the trichomonad species involved these infections are either asymptomatic or can lead to sometimes serious disease. To further study protozoal agents of the order Trichomonadida the establishment of a method to detect trichomonads directly in the tissue, allowing parasite-lesion correlation, is necessary.
View Article and Find Full Text PDFMolecular phylogeny of Trichomonadidae family inferred from ITS-1, 5.8S rRNA and ITS-2 sequences.
Int J Parasitol
July 2004
Centro de Biologia Genômica e Molecular, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
The Trichomonads have been the subject of several molecular studies that reported some discrepancies both at the lower and higher taxonomic levels. The purpose of this study was to make an extensive phylogenetic analysis of the Trichomonadidae using ITS-1/5.8S/ITS-2 sequences, to better understand its phylogeny and the usefulness of this marker.
View Article and Find Full Text PDFPhylogenetic relationships of class II fumarase genes from trichomonad species.
Mol Biol Evol
August 2001
Institut Pasteur, INSERM Unité 547, Lille, France.
Class II fumarase sequences were obtained by polymerase chain reaction from five trichomonad species. All residues known to be highly conserved in this enzyme were present. Nuclear run-on assays showed that one of the two genes identified in Tritrichomonas foetus was expressed, whereas no fumarase transcripts were detected in the related species Trichomonas vaginalis.
View Article and Find Full Text PDFCentrin protein and genes in Trichomonas vaginalis and close relatives.
J Eukaryot Microbiol
May 2000
Biologie des Protistes, UPRESA 6023, Université Blaise Pascal de Clermont-Ferrand, Aubiere, France.
Anti-centrin monoclonal antibodies 20H5 and 11B2 produced against Clamydomononas centrin decorated the group of basal bodies as well as very closely attached structures in all trichomonads studied and in the devescovinids Foaina and Devescovina. Moreover, these antibodies decorated the undulating membrane in Trichomonas vaginalis, Trichomitus batrachorum, and Tritrichomonas foetus, and the cresta in Foaina. Centrin was not demonstrated in the dividing spindle and paradesmosis.
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