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http://dx.doi.org/10.1002/jps.3030481109 | DOI Listing |
Anal Chem
January 2025
Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China.
The rapid, sensitive, and accurate detection of paralytic shellfish toxins (PSTs), such as saxitoxin (STX), is critical for protecting human health due to the frequent occurrence of toxic red tides. In this work, to address the low affinity of traditional mouse monoclonal antibodies (m-mAbs), rabbit monoclonal antibodies (r-mAbs) against STX were produced by a single B-cell sorting culture and a cross-selection strategy. The r-mAbs showed 100-fold improvement in sensitivity (IC = 0.
View Article and Find Full Text PDFACS Synth Biol
January 2025
KAUST Catalysis Center (KCC), Division of Physical Sciences & Engineering, King Abdullah University of Science and Technology, KAUST, Thuwal 23955, Kingdom of Saudi Arabia.
The COVID-19 pandemic has highlighted the critical need for pathogen detection methods that offer both low detection limits and rapid results. Despite advancements in simplifying and enhancing nucleic acid amplification techniques, immunochemical methods remain the preferred methods for mass testing. These methods eliminate the need for specialized laboratories and highly skilled personnel, making home testing feasible.
View Article and Find Full Text PDFAnal Chem
January 2025
Key Laboratory of Laboratory Medicine, Ministry of Education, Zhejiang Provincial Key Laboratory of Medical Genetics, Cixi Biomedical Research Institute, School of Laboratory Medicine and Life sciences, Wenzhou Medical University, Wenzhou 325035, China.
Accurate identification of cancer cells under complex physiological environments holds great promise for noninvasive diagnosis and personalized medicine. Herein, we developed dual-aptamer-based DNA logic-gated series lamp probes (Apt-SLP) by coupling a DNA cell-classifier (DCC) with a self-powered signal-amplifier (SSA), enabling rapid and sensitive identification of cancer cells in a blood sample. DCC is endowed with two extended-aptamer based modules for recognizing the two cascade cell membrane receptors and serves as a DNA logic gate to pinpoint a particular and narrow subpopulation of cells from a larger population of similar cells.
View Article and Find Full Text PDFJ Fluoresc
January 2025
School of Mechanical Engineering, Yeungnam University, Gyeongsan, South Korea.
An eco-friendly nitrogen-passivated carbon dot (N-CDs)-based fluorescent sensor was designed for the selective and sensitive detection of thiophanate-methyl, a widely applied fungicide in agriculture. The synthesized N-CDs exhibited robust fluorescence and remarkable photostability, which contributed to the sensor's performance. Notably, the sensor achieved a detection limit as low as 4.
View Article and Find Full Text PDFClin Chem Lab Med
January 2025
Institute of Clinical Chemistry and Laboratory Medicine, University Medical Center of the Johannes Gutenberg University, Mainz, Germany.
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