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Single-molecule resolution of the conformation of polymers and dendrimers with solid-state nanopores.
Talanta
January 2025
Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences & Chongqing School, University of Chinese Academy of Science, Chongqing, 400714, PR China. Electronic address:
Polymers and dendrimers are macromolecules, possessing unique and intriguing characteristics, that are widely applied in self-assembled functional materials, green catalysis, drug delivery and sensing devices. Traditional approaches for the structural characterization of polymers and dendrimers involve DLS, GPC, NMR, IR and TG, which provide their physiochemical features and ensemble information, whereas their unimolecular conformation and dispersion also are key features allowing to understand their transporting profile in confined ionic nanochannels. This work demonstrates the nanopore approach for the determination of charged homopolymers, neutral block copolymer and dendrimers under distinct bias potentials and pH conditions.
View Article and Find Full Text PDFMultilocus sequencing analysis of the rhizobial symbionts isolated from Acacia salicina (Lindl.) grown in different regions in Tunisia reveals putative novel Bradyrhizobium species.
World J Microbiol Biotechnol
December 2024
Laboratory of Biodiversity and Valorization of Arid Areas Bioresources (BVBAA), Faculty of Sciences of Gabes, University of Gabes Erriadh, 6072, Zrig, Tunisia.
In this study, we investigated various chromosomal and symbiotic markers in 40 bacterial strains that nodulate an invasive alien plant, Acacia salicina Lindl. in Tunisia. Our findings showed that the native rhizobia associated to A.
View Article and Find Full Text PDFElectromigration of Charged Analytes Through Immiscible Fluids in Multiphasic Electrophoresis.
Electrophoresis
January 2025
Department of Bioengineering, University of California, Berkeley, California, USA.
Multiphasic buffer systems have been of greatest interest in electrophoresis and liquid-liquid electrotransfer; this study extends that foundation by exploring the interplay of the geometric and viscous properties of an interleaving oil layer on the electrotransfer of a charged analyte from an aqueous solution into a hydrogel. We utilized finite element analysis to examine two complementary configurations: one being electrotransfer of a charged analyte (protein) in an aqueous phase into a surrounding hydrogel layer and another being electrotransfer of the protein from that originating aqueous phase-through an interleaving oil layer of predetermined viscosity and thickness-and into a surrounding hydrogel layer. Results indicate that the presence of an oil layer leads to increased skew of the injected peak.
View Article and Find Full Text PDFWheat TaPYL9-involved signalling pathway impacts plant drought response through regulating distinct osmotic stress-associated physiological indices.
Plant Biotechnol J
February 2025
State Key Laboratory of North China Crop Improvement and Regulation, Baoding, Hebei, China.
The abscisic acid (ABA) signalling pathway plays a crucial role in plants' response to drought stress. In this study, we aimed to characterize the impact of an ABA signalling module, which consisted of TaPYL9 and its downstream partners in Triticum aestivum, on plant drought adaptation. Our results showed that TaPYL9 protein contains conserved motifs and targets plasma membrane and nucleus after being sorted by the endoplasmic reticulum.
View Article and Find Full Text PDFWide-pore fully porous mixed-mode octyl/pyridyl-bonded silica material with pH-dependent surface charge reversal for high-performance hydrophobic charge-induction chromatography of proteins.
J Chromatogr A
November 2024
Institute of Pharmaceutical Sciences, Pharmaceutical (Bio-)Analysis, University of Tübingen, Auf der Morgenstelle 8, Tübingen 72076, Germany. Electronic address:
Article Synopsis
- Researchers developed a new mixed-mode stationary phase for protein high-performance liquid chromatography (HPLC) by combining octyl and 2-pyridylethyl ligands on silica, aiming to reduce unfavorable interactions seen in standard butyl-bonded silica.
- This method involved creating a dense polymeric siloxane layer on silica, and the performance of this phase was compared to traditional butyl-bonded and mixed-mode octyl/3-aminopropyl silica phases.
- The study found that the new mixed-mode phase produced better protein separation under acidic conditions (pH 3) by reducing retention times and improving peak shapes, thanks to reduced silanophilic interactions.
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