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Thiazole-Formulated Azomethine Compound for Three-Way Detection of Mercury Ions in Aqueous Media and Application in Living Cells.
ACS Omega
July 2022
Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Vellore, Tamil Nadu 632014, India.
Heavy metal ions are extremely poisonous and cause long-term harm to living organisms. Among these ions, mercury is the most toxic metal and has no notorious purpose in the human body. In this regard, an elegant azomethine thiazole compound was synthesized, and it was found to be highly sensitive to three-way detection of mercury ions with detection limits of 0.
View Article and Find Full Text PDFEffects of temperature fluctuation on endocrine disturbance of grass carp Ctenopharyngodon idella under mercury chloride stress.
Chemosphere
January 2021
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223, China.
Mercury (Hg) is considered to be one of the most toxic and ubiquitously distributed metals in the aquatic system. Meanwhile, the temperature increase of water bodies due to global climatic changes, may affect ecosystems through alterations of the metal properties or by affecting the susceptibility of organisms. To study the physiological stress of mercury chloride on grass carp Ctenopharyngodon idella at different temperatures, we investigated the effects of water temperature and/or mercury chloride (HgCl) on growth performance (SGR-the specific growth rate, HSI-hepato-somatic index, CF-condition factor) and the thyroid hormones levels (T3-triiodothyronine; T4-thyroxine), as well as the expression levels of related genes involved growth and hypothalamus-pituitary-thyroid (HPT) axis.
View Article and Find Full Text PDFRegulation of glutathione-dependent antioxidant defense system of grass carp Ctenopharyngodon idella under the combined stress of mercury and temperature.
Environ Sci Pollut Res Int
January 2021
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223, China.
In this study, we investigated the combined effects of temperatures fluencies and mercury (Hg) on glutathione-dependent antioxidant system in fish, by measuring the oxidative stress indicator (LPO, lipid peroxidation) and the parameters involved in the glutathione-related antioxidant defense system (GPx, glutathione peroxidase; GR, glutathione reductase; GST, glutathione S-transferase; GSH, reduced glutathione), as well as the expression of related genes in grass carp, Ctenopharyngodon idella. Fish (45.37 ± 3.
View Article and Find Full Text PDFDivergent Pair of Ultrasensitive Mechanoelectronic Nanoswitches Made out of DNA.
Anal Chem
July 2019
Key Laboratory of Synthetic and Natural Functional Molecular Chemistry (Ministry of Education), College of Chemistry and Materials Science , Northwest University, Xi'an , Shaanxi 710127 , China.
Mechanoelectronic DNA nanoswitches refer to designed oligonucleotide constructs that are composed of conduction-interrupted duplex stems functionally coupled to ligand recognition motifs; they have been shown to undergo remarkable conduction switching upon binding molecular ligands/analytes. Herein we report a divergent pair of such mechanoelectronic DNA switches, the "signal-on" 3'AA-1 switch and the "signal-off" NB-1 switch, both activated by and responded to mercury ions (Hg) at nM levels. We first investigated their charge transport efficiency at a biochemical level, by studying how distinct base sequence at the switches' central three-way junction and at the recognition motif (capable of forming T-Hg-T metallo-base pairs) influences their overall conductivity.
View Article and Find Full Text PDFElectrogenerated chemiluminescence biosensor for detection of mercury (II) ion via target-triggered manipulation of DNA three-way junctions.
Talanta
March 2019
Synthetic and Natural Functional Molecule Chemistry of Ministry of Education Key Laboratory, College of Chemistry and Materials Science, Northwest University, Xi'an, Shaanxi 710127, PR China.
A new electrogenerated chemiluminescence (ECL) biosensor is fabricated for the determination of mental ion incorporating DNA three-way junction structure (DNA-TWJ). As a model system, Hg was chosen as an analyte. The ECL biosensor was fabricated by covalently coupling Hg special DNA-TWJ tagged with ruthenium (II) complex (Ru) (named TW/Ru-J1) to the surface of glassy carbon electrode that had been covalently modified with 4-aminobenzoic acid via electrochemical oxidations.
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