AI Article Synopsis
- A new murine cell line called SR-4987 was created from long-term bone marrow culture using supernatant from Y-1 cells that produce viral C-particles.
- The SR-4987 line showed characteristics typical of fibroblasts and confirmed mesodermal origin, displaying a tetraploid chromosome number and specific cell cycle distribution.
- This cell line is tumorigenic in syngeneic mice and produces M-CSF, while ongoing studies aim to explore its potential autocrine growth factors and the role of stromal cells in influencing hemopoietic progenitor interactions.
Article Abstract
A new murine cell line designated as SR-4987 was established by treating a long-term bone marrow culture with the supernatant from Y-1 cells which actively produce viral C-particles (MuLV). The line showed a fibroblast-like morphology and its mesodermal origin was confirmed by immunocytochemical staining. Flow cytometric analysis of DNA index evidenced a tetraploid number of chromosomes whereas cell cycle analysis showed 34.8% of cells in S phase and 60.7% in G1. In vitro growth studies demonstrated a population doubling time of 14.7 h, a good plating efficiency (52.3%) and a very poor agar clonogenic capacity (0.6%). SR-4987 was tumorigenic only in syngeneic mice in which sarcomas were induced. The line produced M-CSF in the culture supernatant whereas G-CSF, IL-3 and GM-CSF were not detected. Studies are in progress to assess the production of other cytokines and to verify if same autocrine growth factor is involved in the control of SR-4987 proliferation. Our line provides a further model of stromal cells for studying the interaction between hemopoietic progenitors and their microenvironment, as well as to study factors produced by stromal cells acting as modulators of proliferation and differentiation of related cell populations.
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| http://dx.doi.org/10.1007/BF02525491 | DOI Listing |
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