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Effect of Elevated Temperature on Compressive Strength of MICCP and EICCP Biocemented Mortar.
Mol Biotechnol
January 2025
Department of Civil Engineering, Visvesvaraya National Institute of Technology (VNIT), Nagpur, Maharashtra, 440010, India.
Recently biocementation has got attention of many researchers worldwide as one of the most potent techniques for sustainable construction. Several studies have been carried out worldwide on biocementation by urea hydrolysis. Biocementation by bacterially induced calcium carbonate precipitation by different bacterial species has been among the most widely researched areas in this field.
View Article and Find Full Text PDFInterplay between conformational dynamics and substrate binding regulates enzymatic activity: a single-molecule FRET study.
Chem Sci
January 2025
Department of Chemical and Biological Physics, Weizmann Institute of Science Rehovot 761001 Israel
Proteins often harness extensive motions of domains and subunits to promote their function. Deciphering how these movements impact activity is key for understanding life's molecular machinery. The enzyme adenylate kinase is an intriguing example for this relationship; it ensures efficient catalysis by large-scale domain motions that lead to the enclosure of the bound substrates ATP and AMP.
View Article and Find Full Text PDFCharacteristics of soybean () protein hydrolysate by bromelain and its effect on inflammation and kidney disorders in gentamicin-induced male Wistar rats.
Iran J Basic Med Sci
January 2025
Faculty of Medicine, Universitas Jenderal Achmad Yani, Jalan Terusan Jenderal Sudirman, Cimahi 40531 PO Box 148, Indonesia.
Objectives: Soybeans have various positive effects on health, including anti-inflammatory and preventing kidney damage. There is concern regarding the phytoestrogen content due to the high isoflavone content in soybeans. Various forms of soybean processing have been tried; in this study, the hydrolysis method will be used to obtain the active substance Arginine-Glycine-Aspartate (RGD) tripeptide in soybean protein hydrolyzed by bromelain (SPHB).
View Article and Find Full Text PDFBuilding Localized NADP(H) Recycling Circuits to Advance Enzyme Cascadetronics.
Angew Chem Int Ed Engl
January 2025
University of Oxford, Chemistry, UNITED KINGDOM OF GREAT BRITAIN AND NORTHERN IRELAND.
The catalytic action of enzymes of a cascade trapped within a mesoporous electrode material is simultaneously energized, controlled and observed through the efficient, reversible electrochemical NAD(P)(H) recycling catalyzed by one of the enzymes. In their nanoconfined state, nicotinamide cofactors are tightly channeled current carriers, mediating multi-step reactions in either direction (oxidation or reduction) with a rapid response time. By incorporating a hydrogen‑borrowing enzyme pair, the internal action of which opposes the external voltage bias driving oxidation or reduction, a reduction process can be performed under overall oxidizing conditions, and vice versa.
View Article and Find Full Text PDFAssembly of collagen fibers into contiguous dense and loose regions of subcutaneous fascia.
Connect Tissue Res
January 2025
Graduate School of Engineering, Kogakuin University, Hachioji, Tokyo, Japan.
Objective: This study aimed to investigate the collagen fiber structure of the subcutaneous fascia, a connective tissue layer between the skin and epimysium.
Methods: Fascia samples with varying extensibility were examined using biochemical and microscopic methods.
Results: Loose fascia, the more extensible type, displayed sparsely distributed collagen fibers, while dense fascia showed tightly packed collagen fiber bundles.
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