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Molecular characterization and risk analysis of Giardia duodenalis assemblages in corticosteroid-treated and non-treated patients in Ismailia, Arab Republic of Egypt.
Gut Pathog
December 2024
Department of Parasitology, Faculty of Medicine, Suez Canal University, Ismailia, 41522, Egypt.
Background: Giardia duodenalis (G. duodenalis) is an intestinal protozoan parasite of human and animal hosts. The present study investigated and compared the assemblages of G.
View Article and Find Full Text PDFDevelopment of a "big data" groundwater microbial contamination index and spatial comparisons with enteric infection rates in southern Ontario.
Sci Total Environ
October 2024
School of Environmental Studies, Queen's University, 99 University Ave, Kingston, ON, Canada; Public Health Ontario, 181 Barrie St, Kingston, ON, Canada. Electronic address:
Big data have become increasingly important for policymakers and scientists but have yet to be employed for the development of spatially specific groundwater contamination indices or protecting human and environmental health. The current study sought to develop a series of indices via analyses of three variables: Non-E. coli coliform (NEC) concentration, E.
View Article and Find Full Text PDFTransition to PCR diagnosis of cryptosporidiosis and giardiasis in the Norwegian healthcare system: could the increase in reported cases be due to higher sensitivity or a change in the testing algorithm?
Eur J Clin Microbiol Infect Dis
May 2022
Parasitology, Department of Paraclinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, NMBU Veterinærhøgskolen, Postboks 5003, 1432, Ås, Norway.
Cryptosporidiosis has been a notifiable infection in Norway since 2012 and giardiasis since 1977. For both infections, there has been an increase in notified cases. We used a questionnaire to explore whether this may be associated with implementation of molecular diagnostic methods.
View Article and Find Full Text PDFGene migration of giardiasis in Iran; a microevolutionary scale for reflecting transmission patterns of Giardia lamblia assemblages in symptomatic patients.
Microb Pathog
January 2022
Department of Parasitology and Mycology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Article Synopsis
- The study examines the microevolution of Giardia lamblia, tracking gene migration and transmission between adjacent counties in northwest Iran, specifically focusing on samples from Tabriz and Ardabil cities.
- A total of 33 fecal samples were analyzed for identifying G. lamblia assemblages using PCR-RFLP methods targeting the glutamate dehydrogenase locus, resulting in 81.9% identified as assemblage A (genotype AII) and 9% each for assemblage B (genotype BIII) and a mixed genotype.
- Despite varied gastrointestinal symptoms, genetic analysis revealed low diversity in sub-assemblage AII and indicated ongoing gene flow between populations, suggesting a need for more extensive studies on the genetic
Serglycin-Deficiency Causes Reduced Weight Gain and Changed Intestinal Cytokine Responses in Mice Infected With .
Front Immunol
October 2021
Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.
The proteoglycan serglycin (SG) is expressed by different innate and adaptive immune cells, mast cells, macrophages, neutrophils, and cytotoxic T lymphocytes, where SG contributes to correct granule storage and extracellular activity of inflammatory mediators. Here the serglycin-deficient (SG) mouse strain was used to investigate the impact of SG on intestinal immune responses during infection with the non-invasive protozoan parasite . Young (≈11 weeks old) oral gavage-infected congenic SG mice showed reduced weight gain as compared with the infected SG littermate mice and the PBS-challenged SG and SG littermate mice.
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