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Roles of flgJ in biofilm formation of Vibrio alginolyticus.
J Appl Microbiol
March 2025
Hainan Provincial Key Laboratory for Tropical Hydrobiology and Biotechnology, Hainan University, Haikou 570228 Hainan Province, China.
Aim: This study aimed to investigate the role of two flgJ genes in flagellar assembly and biofilm regulation in Vibrio alginolyticus.
Methods And Results: To investigate the functions of the flgJ, overexpression and gene knockout techniques were employed. Overexpression of flgJ1 enhanced the strain's growth capacity, leading to a rapid bacterial concentration that initiated biofilm formation.
In vitro study of a siRNA delivery liposome constructed with an ionizable cationic lipid.
Zhong Nan Da Xue Xue Bao Yi Xue Ban
October 2024
Department of Pharmaceutical Engineering, Chemistry and Chemical Engineering, Central South University, Changsha 410083.
Objectives: Small interfering RNA (siRNA) can silence disease-related genes through sequence-specific RNA interference (RNAi). Cationic lipid-based liposomes effectively deliver nucleic acids into the cytoplasm but often exhibit significant toxicity. This study aims to synthesize a novel ionizable lipid, Nε-laruoyl-lysine amide (LKA), from natural amino acids, constructed LKA-based liposomes, and perform physicochemical characterization and cell-based experiments to systematically evaluate the potential of these ionizable lipid-based liposomes for nucleic acid delivery.
View Article and Find Full Text PDFNeu1-Deficient Zebrafish Cells Exhibit Reduced Edwardsiella piscicida Infection Due to Altered Lysosomal Exocytosis and Membrane Dynamics.
Fish Shellfish Immunol
March 2025
Department of Food Life Sciences, Faculty of Fisheries, Kagoshima University, Kagoshima, 890-0056, Japan; Course of Biological Science and Technology, The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, 890-0065, Japan. Electronic address:
Edwardsiella piscicida is a Gram-negative intracellular pathogen causing Edwardsiellosis, leading to economic losses in aquaculture. While phagocytosis is its primary infection route, alternative entry pathways remain largely unexplored. Neu1 sialidase, a lysosomal enzyme in glycoconjugate degradation, was investigated for its role in E.
View Article and Find Full Text PDFExplore peptides extracted from gliadin hydrolysates suppressing BACE1 activity and restraining Aβ protein deposition.
Int J Biol Macromol
March 2025
Institute of Translational Medicine and New Drug Development, China Medical University, Taichung 404333, Taiwan; Department of Biomedical Sciences and Engineering, Tzu Chi University, Hualien 970374, Taiwan. Electronic address:
Alzheimer's Disease (AD) constitutes approximately 70 % of dementia cases and is the most prevalent form of dementia. Current therapeutic options, including acetylcholinesterase inhibitors and N-methyl d-aspartate (NMDA) receptor antagonists, provide symptomatic relief but do not cure the disease and often come with side effects. The primary pathological features of AD are amyloid plaques and neurofibrillary tangles, with amyloid plaques formed by the abnormal accumulation of Amyloid-β (Aβ).
View Article and Find Full Text PDFIdentification and functional regulation of two alternative splicing isoforms of the Uhrf2 gene in Miichthys miiuy.
Dev Comp Immunol
March 2025
Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China; National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, China. Electronic address:
Alternative splicing can produce a variety of splicing isoforms to increase protein diversity, participate in the regulation of gene expression and the occurrence and development of diseases, and thus play an important role in innate immunity. Ubiquitin like with PHD and ring finger domains 2 (Uhrf2) protein is associated with cell proliferation, inflammation, tumors and cancer, and is currently the focus of medical immunology research, but there is little research on alternative splicing of the Uhrf2 gene. In this study, we identified two different splice isoforms of Uhrf2 in Miichthys miiuy through Sanger sequencing, dual-luciferase reporter gene assay, qRT-PCR, subcellular localization experiments, and named them Uhrf2-α and Uhrf2-β.
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