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Functional Characterization of Two Polymerizing Glycosyltransferases for the Addition of -Acetyl-d-galactosamine to the Capsular Polysaccharide of .
Biochemistry
January 2025
Department of Chemistry, Texas A&M University, College Station, Texas 77842, United States.
The exterior surface of the human pathogen is coated with a capsular polysaccharide (CPS) that consists of a repeating sequence of 2-5 different sugars that can be modified with various molecular decorations. In the HS:2 serotype from strain NCTC 11168, the repeating unit within the CPS is composed of d-ribose, -acetyl-d-galactosamine, and a d-glucuronic acid that is further amidated with either serinol or ethanolamine. The d-glucuronic acid moiety is also decorated with d-glycero-l-gluco-heptose.
View Article and Find Full Text PDFIdentification of the Polymerizing Glycosyltransferase Required for the Addition of d-Glucuronic Acid to the Capsular Polysaccharide of .
Biochemistry
January 2025
Department of Chemistry, Texas A&M University, College Station, Texas 77843, United States.
is the leading cause of food poisoning in Europe and North America. The exterior surface of this bacterium is encased by a capsular polysaccharide that is attached to a diacyl glycerol phosphate anchor via a poly-Kdo (3-deoxy-d--oct-2-ulosinic acid) linker. In the HS:2 serotype of NCTC 11168, the repeating trisaccharide consists of d-ribose, -acetyl-d-glucosamine, and d-glucuronate.
View Article and Find Full Text PDF4-O-Methylglucuronoxylan from Hygrophila Ringens var. Ringens Seeds: Chemical Composition and Anti-Inflammatory Activity.
Macromol Biosci
January 2025
Friedrich Schiller University Jena, Institute for Organic Chemistry and Macromolecular Chemistry, Center of Excellence for Polysaccharide Research, Humboldtstraße 10, D-07743, Jena, Germany.
Hygrophila ringens var. ringens is a medicinal plant of the Acanthaceae family. A soluble polysaccharide is extracted from H.
View Article and Find Full Text PDFPreparation, characterization and activity verification of xylooligosaccharides from plantain straw.
Heliyon
December 2024
Chongqing Jiangbei District Disease Control Center, Chongqing, 400020, China.
Currently, the utilization value of plantain straw is low. To increase its value, plantain straw was utilized in this study to produce xylooligosaccharides (XOS). XOS were obtained from plantain straw through xylanase hydrolysis.
View Article and Find Full Text PDFAn improved HPAEC-PAD method for the determination of D-glucuronic acid and 4-O-methyl-D-glucuronic acid from polymeric and oligomeric xylan.
BMC Biotechnol
December 2024
Division of Biotechnology, Department of Chemistry, Lund University, PO Box 124, 22100, Lund, Sweden.
Article Synopsis
- Glucuronic acid (GlcA) and its methylated form (MeGlcA) are key components of hardwood xylan that can impact digestibility.
- Current methods for detecting these acids from synthetic substrates are effective, but analyzing them in natural substrates like xylan presents challenges.
- This study proposes new gradient elution protocols using HPAEC-PAD to enhance the separation and quantification of GlcA and MeGlcA in hardwood samples, demonstrating excellent reproducibility and reliable results.
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