AI Article Synopsis
- The study investigates how the sensitivity to estrogen changes with age in female mice, specifically looking at the modulation of proopiomelanocortin (POMC) mRNA by estradiol (E2).
- Young mice showed a significant decrease in POMC mRNA levels with E2 treatment, while older mice did not experience this effect.
- Findings confirm that the ability of E2 to lower POMC mRNA is diminished by middle age, highlighting a potential molecular alteration in the neuroendocrine system as rodents age.
Article Abstract
Altered neuroendocrine sensitivity to estrogen is a characteristic of reproductive aging in female rodents, but its molecular basis is not well understood. The objective of this study was to determine whether altered modulation of hypothalamic proopiomelanocortin (POMC) mRNA by estradiol (E2) is a component of reduced neuroendocrine sensitivity to estrogen in the aging mouse. Young (4 month-old), middle-aged (13 month-old), and old (25 month-old) C57BL/6J mice were ovariectomized, implanted 2 weeks later with Silastic capsules containing E2 or cholesterol (CHOL), and sacrificed 3 days later. Hypothalamic POMC mRNA was measured by solution hybridization/RNase protection, using a RNA probe complementary to a fragment of mouse POMC mRNA. In the group with CHOL implants, POMC mRNA was 36% lower in middle-aged and old mice compared to young mice. E2 treatment reduced POMC mRNA levels by 44% in young mice but failed to lower POMC mRNA in middle-aged and old animals. These results confirm earlier evidence of reduced levels of POMC mRNA in hypothalami of aging rodents and indicate that the ability of E2 to reduce hypothalamic POMC mRNA is lost by middle age.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1159/000126181 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of Illumination Color on Hypothalamic Appetite-Regulating Gene Expression and Glycolipid Metabolism.
Nutrients
December 2024
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Irregular illumination is a newly discovered ambient factor that affects dietary and metabolic processes. However, the effect of the modulation of long-term light exposure on appetite and metabolism remains elusive. Therefore, in this current study, we systematically investigated the effects of up to 8 weeks of exposure to red (RL), green (GL), and white light (WL) environments on appetite, food preferences, and glucose homeostasis in mice on both high-fat and low-fat dietary patterns.
View Article and Find Full Text PDFIdentification of Three Genotypes in Largemouth Bass () and Their Differential Physiological Responses to Feed Domestication.
Animals (Basel)
December 2024
State Key Laboratory of Developmental Biology of Freshwater Fish, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
Diverse feeding habits in teleosts involve a wide range of appetite-regulating factors. As an appetite-suppressing gene, the polymorphisms of in largemouth bass () were validated via sequencing and high-resolution melting (HRM). The frequency distribution of different genotypes were analyzed in two populations, and physiological responses of different genotypes to feed domestication were investigated.
View Article and Find Full Text PDFMedial hypothalamic MC3R signalling regulates energy rheostasis in adult mice.
J Physiol
January 2025
Department of Molecular and Integrative Physiology, University of Illinois Urbana-Champaign, Urbana, IL, USA.
Although mammals resist both acute weight loss and weight gain, the neural circuitry mediating bi-directional defense against weight change is incompletely understood. Global constitutive deletion of the melanocortin-3-receptor (MC3R) impairs the behavioural response to both anorexic and orexigenic stimuli, with MC3R knockout mice demonstrating increased weight gain following anabolic challenges and increased weight loss following anorexic challenges (i.e.
View Article and Find Full Text PDFMicroRNA-34a-5p regulates agouti-related peptide via krüppel-like factor 4 and is disrupted by bisphenol A in hypothalamic neurons.
Gene
February 2025
Departments of Physiology, University of Toronto, Ontario, Canada; Departments of Medicine, University of Toronto, Ontario, Canada. Electronic address:
Obesity is a complex disease marked by increased adiposity and impaired metabolic function. While diet and lifestyle are primary causes, endocrine-disrupting chemicals (EDCs), such as bisphenol A (BPA), significantly contribute to obesity. BPA, found in plastic consumer products, accumulates in the hypothalamus and dysregulates energy homeostasis by disrupting the neuropeptide Y (NPY)/agouti-related peptide (AgRP) and pro-opiomelanocortin (POMC) neurons.
View Article and Find Full Text PDFDysregulated HPA axis during postnatal developmental stages in the BTBR T Itpr3/J mouse: A model of autism spectrum disorder.
Neuropsychopharmacol Rep
March 2025
Department of Anatomy and Cell Biology, Nara Medical University, Kashihara, Japan.
Autism spectrum disorder (ASD) is a neurodevelopmental disorder. Some children with ASD show enhanced cortisol response to stress. BTBR T Itpr3/J (BTBR) mice, an ASD model, display behavior consistent with the three diagnostic categories of ASD and exhibit an exaggerated response to stress in adulthood.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!