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Auranofin disrupts selenium metabolism in Clostridium difficile by forming a stable Au-Se adduct.
J Biol Inorg Chem
May 2009
Burnett School of Biomedical Science, College of Medicine, University of Central Florida, Orlando, FL 32816-2364, USA.
Clostridium difficile is a nosocomial pathogen whose incidence and importance are on the rise. Previous work in our laboratory characterized the central role of selenoenzyme-dependent Stickland reactions in C. difficile metabolism.
View Article and Find Full Text PDFMitochondrial selenium-75 uptake and regulation revealed by kinetic analysis.
Biol Trace Elem Res
July 1995
Department of Chemistry, Indian Institute of Technology, Madras, India.
Uptake of Na2(75)SeO3 by mitochondria of the larvae of the insect C. cephalonica reared at different dietary selenium (Se) levels revealed: 1. A proportional increase in the uptake with externally added Na2(75)SeO3 in concentrations upto 25.
View Article and Find Full Text PDFIncorporation of selenium-75 into seminal plasma and spermatozoa of the bull.
Andrologia
March 1991
Department of Anatomy, Veterinary Research Station, University of Kuopio, Finland.
75Se was given intravenously into 5 bulls. Multiple blood and semen samples were taken and during slaughter 5, 10, 15, 20 and 80 days later samples of various reproductive and other organs were collected. After injection, 75Se in blood reached a peak at 6 h followed by a rapid decline.
View Article and Find Full Text PDFIn a prospective evaluation of patients suspected of having chronic pancreatitis, synthesis of pancreatic enzymes was measured by means of the incorporation of selenium-75-labelled methionine into the proteins of duodenal aspirate during stimulation of pancreatic secretion with secretin (1 CU X kg-1 X h-1) plus cholecystokinin (CCK) (1 IDU X kg-1 X h-1). The rate of pancreatic enzyme synthesis was increased in patients with chronic pancreatitis. Measurement of pancreatic enzyme synthesis was more sensitive in the detection of chronic pancreatitis than either the bicarbonate or the trypsin secretory response to secretin plus CCK.
View Article and Find Full Text PDFIncorporation of selenium-75 into semen and reproductive tissues of bulls and rams.
J Reprod Fertil
November 1983
After intramuscular injections of 500 muCi 75Se, semen was collected periodically over a 63-day period from a selenium-deficient and a selenium-injected ram which were then killed for collection of the reproductive organs for the gel filtration studies. Testes, accessory glands and semen were also obtained from a bull injected intravenously with 75Se. Gel filtration (Sephadex G 150) of ram testis cytosol resulted in 4 75Se peaks (Ve/Vo ratios of 1 X 1, 1 X 5, 2 X 3, 2 X 9).
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