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The method of fixation of cytologic material was modified for a relatively safe immunocytochemical staining with para-aminophenasine (PAPh) in order to provide the intactness of the principal differentiation antigens of lymphocytic surface membranes of human peripheral blood. The method is recommended for practical studies of immunophenotypic lymphocyte subpopulations in HIV-infected subjects and patients with AIDS and other infectious diseases.
View Article and Find Full Text PDFNew immunoenzyme-cytochemical stainings for the in situ detection of epitope specificity and isotype of antibody forming B cells in experimental and natural (auto) immune responses in animals and man.
J Immunol Methods
June 1992
Department of Immunology and Medical Microbiology, TNO Medical Biological Laboratory, Rijswijk, Netherlands.
Pituitary glands from 6-month-old sexually immature female rainbow trout, Salmo gairdneri, were kept in organ culture for 48 or 72 h. Certain groups of pituitaries were cultivated for 48 h on either control medium or medium with 17 alpha-methyltestosterone (MT), or with estradiol-17 beta (E2) in concentrations of 8.5 X 10(-7) M.
View Article and Find Full Text PDFImmunocytochemical evidence for the association of neurophysin-I with vasopressin and neurophysin-II with oxytocin in the pig hypothalamus.
Neurosci Lett
December 1976
Postgraduate School of Obstetrics and Gynaecology, University of Auckland, Auckland New Zealand.
Antisera raised against oxytocin, [8-arginine]-vasopressin, porcine neurophysin-I and porcine neurophysin-II were used to localize the neurohypophysial hormones and neurophysins in the paraventricular nucleus (PVN) of the pig using immunoenzyme cytochemical techniques. In sagittal section, the dorsolateral part of the PVN appeared as a densely populated group of cells which stained heavily for neurophysin-I and vasopressin with only a few neurons staining for neurophysin-II and oxytocin.
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