A micronucleus test was conducted on the peripheral blood of mice and rats utilizing acridine orange-coated slides (AO-coated method) after oral administration of benzene. Blood was sampled at 0, 24, 48, and 72 h after administration of benzene at doses of 500, 1000, and 2000 mg/kg in both mice and rats. The highest occurrence of micronucleated reticulocytes (MNRETs) was observed at 48 h after administration in both species. Species differences was found in the frequency of MNRETs, with the number being lower in rats than in mice. The present results indicate that the micronucleus test can easily detect chromosome aberrations in peripheral blood induced by benzene administration in both mice and rats. Furthermore, the AO-coated method used in this study was simpler to perform and allowed for easier detection of the effect than the conventional method.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Preclinical Toxicological Assessment of 2-(Benzoxazol-2-yl)[(2-hydroxynaphthyl)diazenyl]phenol Derivatives for Blue Light and UV Radiation Photoprotection Applications.
J Appl Toxicol
January 2025
Laboratory of Genetic Toxicology, Lutheran University of Brazil (ULBRA), Canoas, Rio Grande do Sul, Brazil.
The widespread use of electronic devices has led to increased blue light exposure, highlighting the need for effective radiation blockers with blue light protection. Two synthetic 2-(2'-hydroxyphenyl)benzoxazole derivatives named azo-4'-benzoxazole and azo-5'-benzoxazole have shown an unprecedented blue light absorption capacity but had not been subjected to a safety evaluation. This study aimed to evaluate the cytotoxic, genotoxic, and mutagenic activities of these compounds.
View Article and Find Full Text PDFInvolvement of nuclear atrophy of binucleated hepatocytes in the large micronucleus formation induced by rat hepatocarcinogen acetamide.
Toxicol Appl Pharmacol
January 2025
Division of Pathology, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki-shi, Kanagawa 210-9501, Japan.
Acetamide is a hepatocarcinogen in rats. We previously revealed that acetamide induces characteristic large micronuclei in rat liver, suggesting the possible involvement of chromosome aberrations in acetamide-induced hepatocarcinogenesis. To elucidate the mechanism of large micronuclei formation, in this study we examined time-dependent changes in rat hepatocytes after administration of acetamide.
View Article and Find Full Text PDFAlliumcepa exposed to Nativo® fungicide with and without swine liver enzyme biotransformation: A comparative genotoxicity study.
Chemosphere
January 2025
Université de Lorraine, CNRS, LIEC, F-57000, Metz, France. Electronic address:
The biotransformation of compounds is a critical point in understanding their toxic effects in both toxicology and ecotoxicology. The objective of this study was to evaluate whether there is a difference in the phytogenotoxic potential of the commercial fungicide Nativo® between non-biotransformed samples and samples biotransformed by swine liver enzymes. The fungicide Nativo is a mixture of Tebuconazole (TEB; 200 g L) and Trifloxystrobin (TRI; 100 g L).
View Article and Find Full Text PDFThe Impact of the Metabolic Syndrome Severity on the Appearance of Primary and Permanent DNA Damage.
Medicina (Kaunas)
December 2024
Department of Internal Medicine, Division of Endocrinology, University Hospital Centre Zagreb, Croatian Referral Center for Obesity Treatment, Kišpatićeva 12, 10000 Zagreb, Croatia.
The prevalence of metabolic syndrome (MetS) worldwide is rapid and significant on a global scale. A 2022 meta-analysis of data from 28 million individuals revealed a global prevalence of 45.1%, with notably higher rates in the Eastern Mediterranean Region and the Americas, particularly in high-income countries.
View Article and Find Full Text PDFMicronuclei as genotoxicity endpoint applied in the co-culture of two mammalian cell lines.
Mutat Res Genet Toxicol Environ Mutagen
January 2025
Institute of Pharmacology and Toxicology, University of Wuerzburg, Versbacher Strasse 9, 97078 Würzburg, Germany. Electronic address:
There has been a shift from traditional animal models towards alternative methods. While 2D cell culture has a decade long tradition, more advances methods like 3D cultures, organoids, and co-culture techniques, which better mimic in vivo conditions, are not yet well established in every research area. Genotoxicity assessment is an integral part of toxicological testing or regulatory approval of pharmaceuticals and chemicals.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!