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A fast and practicable gas-liquid chromatographic method for the quantitation of non-esterified fatty acids (C14:0-C18:2) from 100 microliter plasma is described. This technique includes extraction, purification using the solvents n-heptane and 0.5 M Na2CO3, and methylation by 0.
View Article and Find Full Text PDFEnzymatic microdetermination of free fatty acids in plasma of animals using paraoxon to prevent lipolysis.
Scand J Clin Lab Invest
May 1985
A simple, sensitive and rapid enzymatic method, manual as well as automated, is described for the determination of free fatty acids in plasma of animals. Spontaneous hydrolysis of esterified fatty acids present in the plasma samples is prevented by adding a specific lipase-esterase inhibitor.
View Article and Find Full Text PDF[Microdetermination of esterified serum cholesterol after saponification].
Ann Biol Clin (Paris)
November 1998
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