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Killing of a Multispecies Biofilm Using Gram-Negative and Gram-Positive Targeted Antibiotic Released from High Purity Calcium Sulfate Beads.
Microorganisms
September 2023
Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH 43210, USA.
Article Synopsis
- Multispecies biofilm orthopedic infections are harder to treat than infections caused by a single species, prompting this study to explore effective treatment options.
- The research tested a combination of high purity antibiotic-loaded calcium sulfate beads (HP-ALCSB) with antibiotics vancomycin and tobramycin against a mixed biofilm of Gram-negative and Gram-positive bacteria.
- Results showed that the combination treatment significantly reduced biofilm growth and surviving resistant bacteria by 50% compared to using the antibiotics separately, suggesting that combined antibiotic therapies may be more beneficial for tackling multispecies biofilms.
A replica plating method for efficient, high-throughput screening of antibiotic gene clusters in bacteria uncovers a holomycin-like cluster in the clinical isolate, Pantoea agglomerans 20KB447973.
J Microbiol Methods
October 2023
Department of Biology, University of Regina, 3737 Wascana Parkway, Regina, Saskatchewan S4S 0A2, Canada. Electronic address:
Bacterial natural products remain a major untapped source for novel antimicrobial scaffolds. Many of these products are encoded by biosynthetic gene clusters (BGCs), which can be identified using functional genomics. We developed a replica-plating approach to quickly screen for antibiotic production mutants from transposon mutant libraries and identify candidate antibiotic BGCs.
View Article and Find Full Text PDFMethodology for Integral Study of Antagonistic Activity of Normal Nasal Microbiota to Select Potential Probiotics Efficient in Eradication of Staphylococcus aureus.
Bull Exp Biol Med
June 2023
Medical Institute, North-Caucasus State Academy, Cherkessk, Karachay-Cherkess Republic, Russia.
The aim of this study was the development of a methodology for the integral study of the antagonistic activity of normal human microbiota against Staphylococcus aureus to enable direct selection (without prior isolation of pure cultures) of potentially highly efficient probiotic preparations. The selection of bacterial representatives of normal human nasal microbiota capable of antagonizing S. aureus was carried out using two complimentary methods: replica-plating and deferred antagonism procedures.
View Article and Find Full Text PDFThe metagenomics of soil bacteria and fungi and the release of mechanical dormancy in hard seeds.
Front Plant Sci
June 2023
College of Forestry, Nanjing Forestry University, Nanjing, Jiangsu, China.
Persistence in the soil is a function of seed physiology, particularly non-germination and inherent lifespan. However, for seeds with mechanical dormancy, non-germination is also a function of the composition and activity of the soil microbiota. We attempted to screen out microorganisms in the soil that can specifically and rapidly decompose the hard fruit pericarps of Maxim.
View Article and Find Full Text PDFHigh-Throughput Screening for Thermostable Polyester Hydrolases.
Methods Mol Biol
November 2022
Department of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, Greifswald, Germany.
Due to the promise of more sustainable recycling of plastics through biocatalytic degradation, the search for and engineering of polyester hydrolases have become a thriving field of research. Furthermore, among other methods, halo formation assays have become popular for the detection of polyester-hydrolase activity. However, established halo-formation assays are limited in their ability to screen for thermostable enzymes, which are particularly important for efficient plastic degradation.
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