High-cell-density cultivations of Escherichia coli in glucose-mineral-salt media produce more than 100 g dry cells litre-1 in special fed-batch modes with feeding of glucose and ammonia only. The specific growth rate can be adjusted to allow optimum recombinant protein generation.
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| http://dx.doi.org/10.1016/s0958-1669(05)80142-9 | DOI Listing |
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