A method is described for the isolation of nuclei and nucleoli from ungerminated pea embryos. Electron micrographs of the nucleolar preparation indicate a high degree of purity but only a partial preservation of composition, because of a loss of material from the nucleoli which occurs during the isolation procedure.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2229752PMC
http://dx.doi.org/10.1083/jcb.6.1.53DOI Listing

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