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J Lab Autom
June 2014
Department of Agricultural and Biosystems Engineering, University of Arizona, Tucson, AZ, USA Department of Aerospace and Mechanical Engineering, University of Arizona, Tucson, AZ, USA Biomedical Engineering and Bio5 Institute, University of Arizona, Tucson, AZ, USA
This study demonstrates a low-cost, portable diagnostic system for rapid antimicrobial susceptibility testing in resource-limited settings. To determine the antimicrobial resistance phenotypically, the growth of pathogens in microwell arrays is detected under different antibiotic conditions. The use of a colorimetric cell viability reagent is shown to significantly improve the sensitivity of the assay compared with standard absorbance spectroscopy.
View Article and Find Full Text PDFWorld J Gastroenterol
October 2012
Animal Experiment Centers, PLA General Hospital, Beijing 100853, China.
Aim: To study the effect of H2 gas on liver injury in massive hepatectomy using the intermittent Pringle maneuver in swine.
Methods: Male Bama pigs (n = 14) treated with ketamine hydrochloride and Sumianxin II as induction drugs followed by inhalation anesthesia with 2% isoflurane, underwent 70% hepatotectomy with loss of bleeding less than 50 mL, and with hepatic pedicle occlusion for 20 min, were divided into two groups: Hydrogen-group (n = 7), the pigs with inhalation of 2% hydrogen by the tracheal intubation during major hepatotectomy; contrast-group (n = 7), underwent 70% hepatotectomy without inhalation of hydrogen. Hemodynamic changes and plasma concentrations of alanine aminotransferase (ALT), aspartate aminotransferase (AST), hyaluronic acid (HA), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and malondialdehyde (MDA) in liver tissue were measured at pre-operation, post-hepatotectomy (PH) 1 h and 3 h.
Appl Physiol Nutr Metab
December 2011
Department of Kinesiology, University of Waterloo, Waterloo, ON N2L3G1 Canada.
This study investigated the hypothesis that adaptations would occur in the sarcoplasmic reticulum in vastus lateralis soon after the onset of aerobic-based training consistent with reduced Ca²⁺-cycling potential. Tissue samples were extracted prior to (0 days) and following 3 and 6 days of cycling performed for 2 h at 60%-65% of peak aerobic power (VO₂(peak)) in untrained males (VO₂(peak) = 47 ± 2.3 mL·kg⁻¹·min⁻¹; mean ± SE, n = 6) and assessed for changes (nmol·mg protein⁻¹·min⁻¹) in maximal Ca²⁺-ATPase activity (V(max)), Ca²⁺-uptake, and Ca²⁺-release (phase 1 and phase 2) as well as the sarcoplasmic (endoplasmic) reticulum Ca²⁺-ATPase (SERCA) isoforms.
View Article and Find Full Text PDFClin Chem Lab Med
October 2011
Department of Laboratory Medicine, Medical University of Vienna and the General Hospital of the City of Vienna, Vienna, Austria.
Background: Urinary iodide concentration (UIC) is useful to evaluate nutritional iodine status. In clinical settings UIC helps to exclude blocking of the thyroid gland by excessive endogenous iodine, if diagnostic or therapeutic administration of radio-iodine is indicated. Therefore, this study established a simple test for the measurement of UIC.
View Article and Find Full Text PDFPoult Sci
December 2010
Faculty of Health Sciences, University of Ljubljana, Ljubljana, Slovenia.
A divergent selection experiment was conducted for 8-wk BW in chickens. At 3, 6, 9, and 12 wk of age, samples of pectoralis profundus (PP) and biceps femoris (BF) muscles from fast-growing and slow-growing lines were used to estimate the enzyme activities and muscle fiber diameter. Microphotometric measurements made in situ of succinate dehydrogenase (SDH, EC 1.
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