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Biosynthesis of Bacteriochlorophylls and Bacteriochlorophyllides in Escherichia coli.
Biotechnol Bioeng
December 2024
Department of Microbiology, Hubei Key Laboratory of Cell Homeostasis, College of Life Science, TaiKang Center for Life and Medical Sciences, Wuhan University, Wuhan, China.
Photosynthesis, the most important biological process on Earth, converts light energy into chemical energy with essential pigments like chlorophylls and bacteriochlorophylls. The ability to reconstruct photosynthesis in heterotrophic organisms could significantly impact solar energy utilization and biomass production. In this study, we focused on constructing light-dependent biosynthesis pathways for bacteriochlorophyll (BChl) a and bacteriochlorophyllide (BChlide) d and c in the model strain Escherichia coli.
View Article and Find Full Text PDF, sp. nov., an Aerobic Anoxygenic Phototroph Resistant to Metalloid Oxyanions Isolated from Gold Mine Tailings.
Microorganisms
October 2024
Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
A polyphasic taxonomic study was carried out on the rod-shaped, orange-pigmented strain C11, isolated from gold mine tailings. Sequencing of the 16S rRNA gene showed a relatedness to , with a 98.4% and 98.
View Article and Find Full Text PDFComparative Study of Spectral and Functional Properties of Wild Type and Double Mutant H(L173)L/I(L177)H Reaction Centers of the Purple Bacterium .
Biochemistry (Mosc)
October 2024
Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.
Previously, we found that in the reaction center (RC) of the purple bacterium , formation of heterodimeric primary electron donor (P) caused by the substitution of His-L173 by Leu, was compensated by the second mutation Ile-L177 - His. Significant changes in the spectral properties, pigment composition, and redox potential of P observed in the H(L173)L RC, are restored to the corresponding characteristics of the native RC in the RC H(L173)L/I(L177)H, with the difference that the energy of the long-wavelength Q optical transition of P increases significantly (by ~75 meV). In this work, it was shown using light-induced difference FTIR spectroscopy that the homodimeric structure of P is preserved in the RC with double mutation with partially altered electronic properties: electronic coupling in the radical-cation of the P dimer is weakened and localization of the positive charge on one of its halves is increased.
View Article and Find Full Text PDFSpectral modulation of B850 bacteriochlorophyll a in light-harvesting complex 2 from purple photosynthetic bacterium Thermochromatium tepidum by detergents and calcium ions.
Biochim Biophys Acta Bioenerg
November 2024
Graduate School of Agriculture, Kobe University, Kobe 657-8501, Japan.
Spectral variations of light-harvesting (LH) proteins of purple photosynthetic bacteria provide insight into the molecular mechanisms underlying spectral tuning of circular bacteriochlorophyll (BChl) arrays, which play crucial roles in photoenergy conversion in these organisms. Here we investigate spectral changes of the Q band of B850 BChl a in LH2 protein from purple sulfur bacterium Thermochromatium tepidum (tepidum-LH2) by detergents and Ca. The tepidum-LH2 solubilized with lauryl dimethylamine N-oxide and n-octyl-β-D-glucoside (LH2 and LH2, respectively) exhibited blue-shift of the B850 Q band with hypochromism compared with the tepidum-LH2 solubilized with n-dodecyl-β-D-maltoside (LH2), resulting in the LH3-like spectral features.
View Article and Find Full Text PDFProbing ligands to reaction centers to limit the photocycle in photosynthetic bacterium Rubrivivax gelatinosus.
J Photochem Photobiol B
August 2024
Institute of Medical Physics, University of Szeged, Korányi Fasor 9, Szeged 6720, Hungary. Electronic address:
Light-induced electron flow between reaction center and cytochrome bc complexes is mediated by quinones and electron donors in purple photosynthetic bacteria. Upon high-intensity excitation, the contribution of the cytochrome bc complex is limited kinetically and the electron supply should be provided by the pool of reduced electron donors. The kinetic limitation of electron shuttle between reaction center and cytochrome bc complex and its consequences on the photocycle were studied by tracking the redox changes of the primary electron donor (BChl dimer) via absorption change and the opening of the closed reaction center via relaxation of the bacteriochlorophyll fluorescence in intact cells of wild type and pufC mutant strains of Rubrivivax gelatinosus.
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