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Cyclodextrin-Cholesterol Alone or in Combination With Cyclodextrin-Vitamin E Improves Bull Sperm Cryopreservation in a Soybean Lecithin Extender.
Reprod Domest Anim
October 2024
Department of Veterinary Sciences, Laboratoire Environnement, Santé et Production Animale (LESPA), Institute of Veterinary Sciences and Agronomic Sciences, University of Batna 1, Batna, Algeria.
Combining cholesterol-loaded methyl-β-cyclodextrin (CD-CHL) with vitamin E-loaded methyl-β-cyclodextrin (CD-Vit E) to combat cold shock and oxidative stress during sperm cryopreservation in soybean lecithin extenders remains unexplored. Thus, the current study aimed to investigate the effect of treating bull sperm with CD-CHL and CD-Vit E prior to cryopreservation in a soybean lecithin extender. Sperm collected from eight fertile bulls were pooled and split into six aliquots.
View Article and Find Full Text PDFAn L-type lectin receptor-like kinase promotes starch accumulation during rice pollen maturation.
Development
March 2021
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China
Starch accumulation is key for the maturity of rice pollen grains; however, the regulatory mechanism underlying this process remains unknown. Here, we have isolated a male-sterile rice mutant, (), which produces nonviable pollen grains with defective starch accumulation. Functional analysis revealed that encodes an active L-type lectin receptor-like kinase (L-LecRLK).
View Article and Find Full Text PDFSynthesis of a Hexavalent Betulinic Acid Derivative as a Hemagglutinin-Targeted Influenza Virus Entry Inhibitor.
Mol Pharm
July 2020
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
Naturally occurring pentacyclic triterpenes, such as betulinic acid (BA) and its derivatives, exhibit various pharmaceutical activities and have been the subject of great interest, in particular for their antiviral properties. Here, we found a new anti-influenza virus conjugate, hexakis 6-deoxy-6-[4--(3β-hydroxy-lup-20(29)-en-28-oate)aminomethyl-1-1,2,3-triazol-1-yl]-2,3-di--acetyl-α-cyclodextrin (CYY1-11, ), in a mini library of pentacyclic triterpene-cyclodextrin conjugates by performing a cell-based screening assay and then exploring the underlying mechanisms. Our results showed that conjugate possessed a high-level activity against the influenza virus A/WSN/33 with an IC value of 5.
View Article and Find Full Text PDFWheat germ agglutinin is a biomarker of whole grain content in wheat flour and pasta.
J Food Sci
March 2020
Nutrition & Metabolism Center, Children's Hospital Oakland Research Inst., 5700 Martin Luther King, Jr. Way, Oakland, CA, 94609, U.S.A.
When consumed as whole grain, wheat has a high nutrient density that contributes to a healthy diet. Yet, products labeled as whole wheat can still contain a substantial amount of refined grain leading to the confusion for consumers, so a method was designed to determine the whole grain status within wheat-based foods. Wheat germ agglutinin (WGA), a lectin found in the germ tissue of wheat kernels, was evaluated as a biomarker of whole grain wheat.
View Article and Find Full Text PDFControllable Display of Sequential Enzymes on Yeast Surface with Enhanced Biocatalytic Activity toward Efficient Enzymatic Biofuel Cells.
J Am Chem Soc
February 2020
Institute for Chemical Biology & Biosensing, and College of Life Sciences , Qingdao University, 308 Ningxia Road , Qingdao 266071 , P. R. China.
A precisely localized enzyme cascade was constructed by integrating two sequential enzymes, glucoamylase (GA) and glucose oxidase (GOx), on a yeast cell surface through an a-agglutinin receptor as the anchoring motif with cohesin-dockerin interaction. The overall catalytic activities of the combinant strains were significantly dependent on the assembly method, enzyme molecular size, enzyme order, and enzyme stoichiometry. The combinant strain with GA-DocC initially bound scaffoldin prior to GOx-DocT exhibited a higher overall reaction rate.
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