The genetic determinant coding for F165(1) fimbriae was cloned from the chromosome of the porcine Escherichia coli wild-type strain 4787 (O115:K-:H51:F165). The fimbrial determinant was further subcloned into the BamHI site of pACYC184 and a restriction map was established. On Southern hybridization, identity between the chromosomally encoded prs-like determinant of strain 4787 and its cloned counterparts was demonstrated. The cloned F165(1) fimbriae and those of the wild-type strain possessed a major protein subunit of molecular mass 18.5 kDa. Strains expressing F165(1) fimbriae were detected using an F165-specific polyclonal antiserum and caused mannose-resistant haemagglutination and agglutination of Forssman latex beads. Antiserum against the cloned F165(1) fimbriae recognized a 18.5 kDa band in the parent strain 4787.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1099/00221287-138-7-1495 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Monitoring F1651 P-like fimbria expression at the single-cell level reveals a highly heterogeneous phenotype.
Infect Immun
May 2015
Groupe de Recherche sur les Maladies Infectieuses du Porc (GREMIP) and Centre de Recherche en Infectiologie Porcine (CRIP), Faculté de Médecine Vétérinaire, Université de Montréal, St-Hyacinthe, Canada
F1651 and the pyelonephritis-associated pili (Pap) are two members of the type P family of adhesive factors. They play a key role in establishing disease caused by extraintestinal pathogenic Escherichia coli (ExPEC) strains in animals and humans. Both F1651 and Pap are under the control of an epigenetic and reversible switch that defines the number of fimbriated (ON) and afimbriated (OFF) cells within a clonal population.
View Article and Find Full Text PDFLrp-DNA complex stability determines the level of ON cells in type P fimbriae phase variation.
Mol Microbiol
September 2011
Groupe de Recherche sur les Maladies Infectieuses du Porc and Centre de Recherche en Infectiologie Porcine, Université de Montréal, Saint-Hyacinthe, Quebec, Canada.
F165(1) and the pyelonephritis-associated pili (Pap) are two members of the type P family of adhesive factors that play a key role in the establishment of disease caused by extraintestinal Escherichia coli (ExPEC) strains. They are both under the control of an epigenetic and reversible switch that defines the number of fimbriated (ON) and afimbriated (OFF) cells within a clonal population. Our present study demonstrates that the high level of ON cells found during F165(1) phase variation is due to altered stability of the DNA complex formed by the leucine-responsive regulatory protein (Lrp) at its repressor binding sites 1-3; after each cell cycle, complex formation is also modulated by the local regulator FooI (homologue to PapI) which promotes the transit of Lrp towards its activator binding sites 4-6.
View Article and Find Full Text PDFEffects of DNA supercoiling and topoisomerases on the expression of genes coding for F165(1), a P-like fimbriae.
FEMS Microbiol Lett
December 2007
Département de pathologie et microbiologie, Université de Montréal, St-Hyacinthe, QC, Canada.
Pathogenic Escherichia coli 4787 (O115:KV165) causes septicemia in pigs and expresses the fimbriae F165(1) encoded by the foo operon that belongs to the P fimbrial family. fooI and fooB, encoding specific foo regulators, are divergently transcribed; their intergenic region is responsible for the regulation of foo expression. The role of global and local supercoiling (transcription-induced supercoiling within the intergenic region) on the regulation of foo expression was investigated.
View Article and Find Full Text PDFInfluence of L-leucine and L-alanine on Lrp regulation of foo, coding for F1651, a Pap homologue.
J Bacteriol
December 2004
Département de Pathologie et Microbiologie, Université de Montréal, C.P. 5000, St-Hyacinthe, QC, Canada J2S 7C6.
The foo operon encodes F165 1 fimbriae that belong to the P-regulatory family and are synthesized by septicemic Escherichia coli. Using an Lrp-deficient host and the lrp gene cloned under the arabinose pBAD promoter, we demonstrated that foo was transcribed proportionally to the amount of Lrp synthesized. L-leucine and L-alanine decreased drastically the steady-state transcription of foo and modified phase variation, independently of the presence of FooI.
View Article and Find Full Text PDFMolecular characterization of extraintestinal pathogenic Escherichia coli (ExPEC) pathogenicity islands in F165-positive E. coli strain from a diseased animal.
FEMS Microbiol Lett
September 2004
Groupe de Recherche sur les Maladies Infectieuses du Porc, Université de Montréal, Faculté de Médecine Vétérinaire, C.P. 5000, Saint-Hyacinthe, Qué. J2S 7C6, Canada.
Septicemic Escherichia coli 4787 (O115: K-: H51: F165) of porcine origin possess gene clusters related to extraintestinal E. coli fimbrial adhesins. This strain produces two fimbriae: F165(1) and F165(2).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!