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An innovative LC-MS/MS-based method for determining CYP 17 and CYP 19 activity in the adipose tissue of pre- and postmenopausal and ovariectomized women using 13C-labeled steroid substrates.
J Clin Endocrinol Metab
April 2014
Department of Obstetrics and Gynecology (T.K., A.T., N.T.), Toho University Medical Center Sakura Hospital, Chiba 285-8741, Japan; Department of Analytical Research (S.H., Y.W., H.M., M.O.), ASKA Pharma Medical Co Ltd, Kawasaki 213-8522, Japan; Department of Urology (Y.S.), Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan; and Department of Clinical Analysis (K.Y.), Tohoku Pharmaceutical University, Sendai 981-8558, Japan.
Context: Does adipose tissue produce steroid hormones like an endocrine organ?
Object: To clarify whether adipose tissue produces sex steroid hormone like an endocrine organ, we estimated several key steroid hormone levels, as well as CYP17 and CYP19 activity, in ovariectomized, pre- and postmenopausal women by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
Subjects And Methods: The subjects were 19 premenopausal (n = 12), postmenopausal (n = 4), and ovariectomized women (n = 3) aged 27-68 years. Serum, visceral adipose and sc adipose samples were taken from these subjects and stored at -70°C.
Three groups of patients with morbid fear neurosis, dissimilar in prevailing of either adrenaline or noradrenaline excretion, were identified using the complex method of sympathoadrenal system (SAS) examination. The test with 0.1 g L-DOPA loading demonstrated the high rate of synthesis and high reactivity of SAS in the first two groups with the adrenal type of SAS functioning as well as an increased hydroxylation in the course of synthesis of catecholamines.
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