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A novel method for expressing and purifying large quantities of functional and stable human voltage-gated proton channel (hH1).
Protein Sci
February 2025
Cell Physiology and Molecular Biophysics Department, Center for Membrane Protein Research, Texas Tech University Health Sciences Center, Lubbock, Texas, USA.
Purifying membrane proteins has been the limiting step for studying their structure and function. The challenges of the process include the low expression levels in heterologous systems and the requirement for their biochemical stabilization in solution. The human voltage-gated proton channel (hH1) is a good example of that: the published protocols to express and purify hH1 produce low protein quantities at high costs, which is an issue for systematically characterizing its structure and function.
View Article and Find Full Text PDFExtraction and purification of Ophiopogon japonicus polysaccharides, structural characterization, biological activity, safety evaluation, chemical modification and potential applications: A review.
Int J Biol Macromol
January 2025
College of Pharmacy, Shaanxi University of Chinese Medicine, Shiji Ave., Xi'an-xianyang New Economic Zone, Shaanxi Province 712046, People's Republic of China. Electronic address:
Ophiopogonis japonicus is a famous medicinal plant in China with a long history of medicinal and food origin. It contains various chemical components, such as polysaccharides, steroidal saponins, alkaloids, flavonoids, etc. According to traditional Chinese medicine (TCM) theory, it has the efficacy of moistening the lungs and nourishing the yin, benefiting the stomach by generating fluids, and clearing the heart to get rid of vexation.
View Article and Find Full Text PDFA review of pharmacological mechanisms, challenges and prospects of macromolecular glycopeptides.
Int J Biol Macromol
January 2025
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, PR China. Electronic address:
Macromolecular glycopeptides are natural products derived from various sources, distinguished by their structural diversity, multifaceted biological activities, and low toxicity. These compounds exhibit a wide range of biological functions, such as immunomodulation, antitumor effects, anti-inflammatory properties, antioxidant activity, and more. However, limited understanding of natural glycopeptides has hindered their development and practical application.
View Article and Find Full Text PDFDevelopment, optimization and validation of a highly sensitive and selective method for the determination of PFAS in animal-based food.
Chemosphere
January 2025
Chemical and Veterinary Analytical Institute Muensterland-Emscher-Lippe, Joseph-König-Str. 40, 48147, Muenster, Germany.
Perfluorocarboxylic acids and perfluorosulfonic acids accumulate in food webs, thus posing a serious threat to food safety. The European Food Safety Authority (EFSA) derived a tolerable weekly intake (TWI) of 4.4 ng/kg body weight for the sum of the four so-called EFSA-PFAS in 2020.
View Article and Find Full Text PDFSynergistic Removal of Rare Earth Elements from Radioactive Molten Salt via Electrodeposition and Adsorption.
Inorg Chem
January 2025
Jiangxi Province Key Laboratory of Nuclear Physics and Technology, East China University of Technology, Nanchang 330013, China.
Recycling waste salt in the dry reprocessing of nuclear fuel and reducing electric energy consumption in the electrorefining process are crucial steps toward addressing significant challenges in this field. The present study proposes a novel approach to purify waste salt by selectively adsorbing excessive fission products using 5A molecular sieves (5A), based on the principles of electrorefining, with the ultimate aim of achieving sustainable development in nuclear fuel. First, Lutetium (Lu)-Bi alloy was synthesized through constant potential electrolysis in the LiCl-KCl-LuCl melt, resulting in a 90.
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