AI Article Synopsis
- Successful receptor-mediated gene delivery often struggles with getting genetic material out of endosomes.
- Researchers used modified defective adenovirus particles to improve this process, achieving over 90% expression of the beta-galactosidase gene in cultured cells.
- The method allows for the delivery of larger DNA molecules, as the gene isn’t integrated into the adenovirus genome, demonstrating the potential for a 48-kilobase cosmid DNA delivery.
Article Abstract
One limit to successful receptor-mediated gene delivery is the exit of the endocytosed material from the endosome. We demonstrate here the delivery of marker genes to tissue culture cells using a modification of the receptor-mediated gene delivery technique that exploits the endosomolytic activity of defective adenovirus particles. In particular, greater than 90% of the transfected-cell population is found to express a beta-galactosidase gene, and, most importantly, this high level of expression can be obtained with psoralen-inactivated virus particles. Furthermore, because the delivered gene is not carried within the genome of the adenovirus particle, the size constraints are relieved, and we can, therefore, show the delivery of a 48-kilobase cosmid DNA molecule.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC49444 | PMC |
| http://dx.doi.org/10.1073/pnas.89.13.6094 | DOI Listing |
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