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Antibiotics-Free Steady Bioproduction of Valuable Chemicals from Organic Wastes by Engineered through Targeted Gene Integration.
ACS Synth Biol
December 2024
CAS Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Engineering, University of Science & Technology of China, Hefei 230026, China.
Bioproduction of chemicals by using engineered bacteria is promising for a circular economy but challenged the instability of the introduced plasmid by conventional methods. Here, we developed a two-plasmid INTEGRET system to reliably integrate the targeted gene into the genome, making it a powerful strain for efficient and steady bioproduction without requiring antibiotic addition. The INTEGRET system allows for gene insertion at over 75% inserting efficiency and flexibly controllable gene dosages.
View Article and Find Full Text PDFStrategic improvement of Shewanella oneidensis for biocatalysis: Approach to media refinement and scalable application in a microbial electrochemical system.
N Biotechnol
November 2024
Institute of Technical Microbiology, Hamburg University of Technology, Hamburg 21073, Germany. Electronic address:
Microbial electrochemical systems offer a sustainable method for the conversion of chemical energy into electrical energy or hydrogen and the production of valuable compounds, contributing to the development of a bio-based economy. This study aimed to enhance the performance of anodic bioelectrochemical systems by improving the current density of Shewanella oneidensis as a biocatalyst through strain modification and medium refinement. The genetic modification, combining the prophage deletion and overexpression of the speC gene, resulted in a 4.
View Article and Find Full Text PDFUV-C-Activated Riboflavin Crosslinked Gelatin Film with Bioactive Nanoemulsion for Enhanced Preservation of Fresh Beef in Modified Atmosphere Packaging.
Foods
October 2024
INRS Armand-Frappier Health Biotechnology Research Centre, Research Laboratories in Sciences, Applied to Food (RESALA), Canadian Irradiation Centre (CIC), MAPAQ Research Chair in Food Safety and Quality, Institute of Nutrition and Functional Foods (INAF), 531 Des Prairies Blvd, Laval, QC H7V 1B7, Canada.
This study explores a new eco-friendly approach for developing bioactive gelatin films using UV-C irradiation-induced photo-crosslinking. Riboflavin, a food-grade photoinitiator, was selected at an optimal concentration of 1.25% (/) for crosslinking gelatin under UV-C exposure for 4 to 22 min.
View Article and Find Full Text PDFCharacterization of Selected Microalgae Species as Potential Sources of Nutrients and Antioxidants.
Foods
July 2024
Institute of Horticulture, Faculty of Horticulture and Landscape Engineering, Slovak University of Agriculture, Tr. A. Hlinku 2, 94976 Nitra, Slovakia.
Microalgae are exceptional organisms from a nutritional perspective, boasting an array of bioactive compounds that have long justified their incorporation into human diets. In this study, we explored the potential of five microalgae species: sp., , , , and .
View Article and Find Full Text PDFEffects of immersing Japanese quail eggs in various doses of riboflavin on reproductive, growth performance traits, blood indices and economics.
Poult Sci
August 2024
Poultry Department, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt.
This investigation aimed to evaluate the impact of immersion (IM) riboflavin treatment on the hatchability, production efficiency, and carcass characteristics of Japanese quail eggs. A total of 260 eggs of Japanese quail birds were used for hatching and were randomly divided into 4 treatments with 5 replicates (13 eggs/replicate) in a fully randomized design. Hatching eggs were immersed in riboflavin for 2 min before incubation.
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