Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1177/6.3.197 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Halogenated Cholesterol Alters the Phase Behavior of Ternary Lipid Membranes.
J Phys Chem B
January 2025
Department of Chemistry, University of Tennessee, Knoxville, Tennessee 37916, United States.
Eukaryotic plasma membranes exhibit nanoscale lateral lipid heterogeneity, a feature that is thought to be central to their function. Studying these heterogeneities is challenging since few biophysical methods are capable of detecting domains at submicron length scales. We recently showed that cryogenic electron microscopy (cryo-EM) can directly image nanoscale liquid-liquid phase separation in extruded liposomes due to its ability to resolve the intrinsic thickness and electron density differences of ordered and disordered phases.
View Article and Find Full Text PDFBalance between photoreduction efficiency, cofactor affinity, and allosteric coupling of halogenase flavoenzymes.
Photochem Photobiol Sci
December 2024
Biophysical Chemistry and Diagnostics, Department of Chemistry, Bielefeld University, Universitätsstraße 25, 33615, Bielefeld, Germany.
Flavin-dependent halogenases (FDHs) are promising candidates for the sustainable production of halogenated organic molecules by biocatalysis. FDHs require only oxygen, halide and a fully reduced flavin adenine dinucleotide (FADH) cofactor to generate the reactive HOX that diffuses 10 Å to the substrate binding pocket and enables regioselective oxidative halogenation. A key challenge for the application of FDHs is the regeneration of the FADH.
View Article and Find Full Text PDFExpanding the scope of copper artificial metalloenzymes: A potential fluorinase?
J Inorg Biochem
February 2025
EaStCHEM School of Chemistry, Joseph Black Building, Kings Buildings, University of Edinburgh, David Brewster Road, Edinburgh EH9 3FJ, UK. Electronic address:
Biocatalysts for fluorination are rare, and thus of great interest for artificial enzyme design. Biohybrid catalysts including Cu-based DNAzymes and dinucleotide catalysts can catalyse enantioselective electrophilic fluorination of β-ketoesters. Here we report the investigation of Cu-based artificial metalloenzymes as catalysts for electrophilic fluorination reactions.
View Article and Find Full Text PDFEnzyme stabilisation due to incorporation of a fluorinated non-natural amino acid at the protein surface.
Sci Rep
November 2024
Department of Biochemical Engineering, University College London, Gordon Street, London, WC1H 0AH, UK.
Article Synopsis
- Researchers engineered E. coli transketolase enzyme variants to enhance substrate acceptance and thermal stability while investigating their aggregation behavior in complex environments like cell lysates.
- They used NMR techniques with specific fluorine-labelled amino acids to study structural changes and identified that mutations at a solvent-exposed site affected enzyme stability and activity differently.
- Findings showed that one variant increased thermal stability significantly, while another variant prevented aggregation at higher temperatures, revealing distinct temperature-dependent behaviors and implications for enzyme performance.
Controlling achiral and chiral conformations of benzyl alcohol by -halogenation, collisional relaxation and dimerisation.
Phys Chem Chem Phys
October 2024
Institute of Physical Chemistry, University of Goettingen, Tammannstr. 6, 37077 Goettingen, Germany.
-Halogenated benzyl alcohol can exist in two different low energy chiral conformations, only one of them with an OH-X contact (X = Cl, Br, I). A third, achiral conformation is enabled by the halogen substitution. We show by IR spectroscopy in supersonic jets that the achiral monomer is less stable than the chiral conformation with OH-X contact, but both can be produced in similar amounts using helium as a carrier gas.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!