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| http://dx.doi.org/10.1042/bj0680708 | DOI Listing |
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Microbial micronutrient sharing, gut redox balance and keystone taxa as a basis for a new perspective to solutions targeting health from the gut.
Gut Microbes
December 2025
Health, Nutrition & Care (HNC), Dsm-Firmenich, Kaiseraugst, Switzerland.
In health, the gut microbiome functions as a stable ecosystem maintaining overall balance and ensuring its own survival against environmental stressors through complex microbial interaction. This balance and protection from stressors is maintained through interactions both within the bacterial ecosystem as well as with its host. As a consequence, the gut microbiome plays a critical role in various physiological processes including maintaining the structure and function of the gut barrier, educating the gut immune system, and modulating the gut motor, digestive/absorptive, as well as neuroendocrine system all of which are crucial for human health and disease pathogenesis.
View Article and Find Full Text PDFAdapting the Laser-Induced Fluorescence Detection Setup of the Standard Capillary Electrophoresis Equipment to Achieve High-Sensitivity Detection of 2-Aminoacridone Labeled Oligosaccharides.
J Sep Sci
March 2025
Institute of Analytical Chemistry of the Czech Academy of Sciences, Brno, Czech Republic.
The high-sensitivity capabilities of laser-induced fluorescence (LIF) detection continuously promote the development of various labels with different fluorescence properties. However, this strategy also requires the adaptation of existing detection systems to suit the excitation and emission characteristics of novel fluorescent tags. In this study, we adapted the LIF detector of the commercial capillary electrophoresis instrument to the specific fluorescence spectra of 2-aminoacridone labeled human milk oligosaccharides.
View Article and Find Full Text PDFPreparation of mixed-mode hydrophilic particle for efficient separation of common human milk oligosaccharides.
J Chromatogr A
March 2025
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China; National Engineering Technique Research Center for Biotechnology, Nanjing, China; Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing, China. Electronic address:
Human milk oligosaccharides (HMOs) are essential for babies' growth. The most commonly used method for HMOs analysis combines hydrophilic interaction liquid chromatography (HILIC) with ultra-performance liquid chromatography (UPLC). However, high-pressure operation and the cost of commercial HILIC columns limit progress in the HMOs research.
View Article and Find Full Text PDFProbiotic Bifidobacterium bifidum strains desialylate MUC13 and increase intestinal epithelial barrier function.
Sci Rep
March 2025
Department of Biomolecular Health Sciences, Division Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Probiotic bacteria including Bifidobacterial species have the capacity to improve intestinal health, but the underlying molecular mechanisms are often not understood. Bifidobacteria are considered keystone species but have a relatively low abundance in the adult intestinal tract. Bifidobacterium colonization depends on degradation of host-derived carbohydrates, including human milk oligosaccharides and mucin-associated oligosaccharides.
View Article and Find Full Text PDFThe Human Milk Oligosaccharide Lacto-N-Fucopentaose III Conjugated to Dextran Inhibits HIV Replication in Primary Human Macrophages.
Nutrients
March 2025
Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
: Individuals with HIV on combined antiretroviral therapy (ART) with virologic suppression exhibit chronic immune activation and immune dysfunction. Numerous studies have shown that human milk oligosaccharide (HMO) controls the postnatal transmission of HIV-1, but its effect on adult HIV-1 infection is not known. The purpose of this study was to investigate the anti-HIV activity of Lacto-N-fucopentaose III (LNFPIII) in adult blood-borne macrophages.
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