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Quality evaluation of the emergency program of three contemporary cell-washing machines.
Perfusion
May 2019
1 Laboratory for Experimental Cardiac Surgery, Ghent University, Belgium.
This study evaluates the efficiency of emergency programs used by three contemporary cell washers. Both time necessary to process a given amount of blood and quality of the processed blood are investigated. The Fresenius Kabi Smart, Haemonetics Elite and LivaNova Xtra were validated using bovine blood, with a starting hematocrit of 13% or 21%.
View Article and Find Full Text PDFA comparison of red cell recovery between two different methods of red cell washing.
Anesth Analg
December 2003
Department of General Anesthesiology and Clinical Pathology, Cleveland Clinic Foundation, Ohio.
Unlabelled: The success of cell salvage varies depending upon how many shed red blood cells (RBC) are captured from the surgical wound and returned to the patient. Here, the authors hypothesized that pneumatic disk (PD) processing might provide better RBC recovery when compared with traditional Latham bowl (LB) techniques. Comparison of the speed of processing, product hemoglobin and salvage efficiency was made between the two machines when their reservoirs were loaded with blood volumes ranging from 100 mL to 1000 mL.
View Article and Find Full Text PDFUltrafiltration of the waste plasma effluent from cardiopulmonary bypass circuit contents processed with a cell-washing device.
J Extra Corpor Technol
September 1996
University of Nebraska Medical Center, Division of Clinical Perfusion Sciences, Omaha 68198-5155, USA.
Blood conservation methods are commonly practiced throughout most hospitals that conduct cardiothoracic surgery. In an effort to reduce patients' exposure to homologous blood products and due to cost effectiveness of blood conservation techniques, this present study combines autotransfusion of the remaining blood in the extracorporeal circuit and ultrafiltration of the plasma effluent, and describes the resulting product. Seven patients, greater than 19 years of age, requiring cardiopulmonary bypass (CPB) were incorporated into this study.
View Article and Find Full Text PDFBCSH-NIBSC anti-D reference reagent for antiglobulin tests: the in-house assessment of red cell washing centrifuges and of operator variability in the detection of weak, macroscopic agglutination. British Committee for Standards in Haematology. National Institute for Biological Standards and Control.
Transfus Med
June 1993
National Institute for Biological Standards and Control, Potters Bar, U.K.
A batch of an anti-D preparation, reference 91/608, has been prepared for the preparation of red cells weakly sensitized with IgG that can reveal inhibition of the antiglobulin test by one volume of human serum, diluted 1:1000. The preparation provides an objective assessment of red cell washer efficacy and the confidential, in-house assessment of operator variability in detecting weak but definite macroscopic agglutination by blind, replicate tests. Red cell washer efficacy and poor operator reading procedures causing disruption of weak agglutination are two major causes of false-negative antiglobulin tests; neither are adequately detected by the common quality-control procedure of adding strongly IgG-sensitized red cells ('Coombs control cells') to apparently negative antiglobulin tests.
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