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Functional Characterization of Two Polymerizing Glycosyltransferases for the Addition of -Acetyl-d-galactosamine to the Capsular Polysaccharide of .
Biochemistry
January 2025
Department of Chemistry, Texas A&M University, College Station, Texas 77842, United States.
The exterior surface of the human pathogen is coated with a capsular polysaccharide (CPS) that consists of a repeating sequence of 2-5 different sugars that can be modified with various molecular decorations. In the HS:2 serotype from strain NCTC 11168, the repeating unit within the CPS is composed of d-ribose, -acetyl-d-galactosamine, and a d-glucuronic acid that is further amidated with either serinol or ethanolamine. The d-glucuronic acid moiety is also decorated with d-glycero-l-gluco-heptose.
View Article and Find Full Text PDFIdentification of the Polymerizing Glycosyltransferase Required for the Addition of d-Glucuronic Acid to the Capsular Polysaccharide of .
Biochemistry
January 2025
Department of Chemistry, Texas A&M University, College Station, Texas 77843, United States.
is the leading cause of food poisoning in Europe and North America. The exterior surface of this bacterium is encased by a capsular polysaccharide that is attached to a diacyl glycerol phosphate anchor via a poly-Kdo (3-deoxy-d--oct-2-ulosinic acid) linker. In the HS:2 serotype of NCTC 11168, the repeating trisaccharide consists of d-ribose, -acetyl-d-glucosamine, and d-glucuronate.
View Article and Find Full Text PDFMulti-level fingerprint and immune activity evaluation of polysaccharides from Rhodiola rosea L.
Int J Biol Macromol
January 2025
School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Jinan 250355, China. Electronic address:
To establish the quality control method of Rhodiola rosea L., the multi-level fingerprinting profile was established. The quality evaluation of Rhodiola rosea L.
View Article and Find Full Text PDFCadmium promotes hyaluronan synthesis by inducing hyaluronan synthase 3 expression in cultured vascular endothelial cells via the c-Jun N-terminal kinase-c-Jun pathway.
Toxicology
January 2025
Department of Environmental Health, Faculty of Pharmaceutical Sciences, Toho University, 2-1-1 Miyama, Funabashi, Chiba 274-8510, Japan. Electronic address:
Cadmium is a heavy metal risk factor for various cardiovascular diseases, such as atherosclerosis. In atherosclerotic lesions, hyaluronan, a glycosaminoglycan consisting of β4-glucuronic acid-β3-N-acetylglucosamine disaccharides repeats, is highly accumulated, regulating signal transduction, cell migration, and angiogenesis. Hyaluronan is synthesized by hyaluronan synthase (HAS)1-3 in the plasma membrane and secreted into the extracellular space.
View Article and Find Full Text PDFSimultaneous extraction and purification of polysaccharides and proteins from Pleurotus ostreatus using an aqueous two-phase system.
J Food Sci
January 2025
College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing, China.
Pleurotus ostreatus is a nutrient-dense edible fungus renowned for its delicate texture, appealing flavor, and numerous potential health benefits. Simultaneous extraction within the framework of food resource processing facilitates the concurrent isolation and analysis of multiple target compounds. In this study, an ethanol/salt aqueous two-phase system (ATPS) was employed to extract polysaccharides (PS) and proteins from P.
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