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Simple methods to determine the dissociation constant, K.
Mol Cells
October 2024
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, South Korea. Electronic address:
The determination of the dissociation constant (K) is pivotal in biochemistry and pharmacology for understanding binding affinities in chemical reactions, which is crucial for drug development and comprehending biological systems. Here, we introduce a single-molecule fluorescence resonance energy transfer-based method for determining K, alongside the conventional electrophoretic mobility shift assay method of K, offering insights into thermodynamic interactions between proteins and substrates. The single-molecule fluorescence resonance energy transfer approach is highlighted for its ability to accurately measure binding and dissociation kinetics through fluorescence labeling and the intrinsic nature of protein-DNA interactions, representing a significant advancement in the fields of molecular biology and pharmacology.
View Article and Find Full Text PDFSubtyping anti-infliximab antibodies by the homogenous mobility shift assay (HMSA): potential utility in a pharmacokinetic case study.
Pathology
June 2023
Department of Immunology, Canterbury Health Laboratories, Christchurch, New Zealand.
Waldenstrom's Macroglobulinemia and Ascites: A Case Report.
J Blood Med
March 2022
UOC Medicina Interna Fatebenefratelli, Naples, Italy.
Background: Waldenstrom's disease is characterized by the presence of pathological changes in the B lymphocytes that are in the last stages of maturation. One characteristic of WM is the production of an abnormal high amount of IgM and hyper viscosity syndrome. The MW gets worse, symptoms such as fatigue, weight loss, night sweats, fever, recurrent infections and swollen lymph nodes develop in patients who have a known history of MGUS.
View Article and Find Full Text PDFWelfare Assessment: Correspondence Analysis of Welfare Score and Hematological and Biochemical Profiles of Dairy Cows in Sardinia, Italy.
Animals (Basel)
March 2021
Istituto Zooprofilattico Sperimentale della Sardegna "G. Pegreffi", Via Duca degli Abruzzi 8, 07100 Sassari, Italy.
The need for animal welfare definition and assessment is increasing worldwide, and several studies have been conducted to help fill the knowledge gaps regarding the welfare of cattle. However, further studies are needed to provide valid synthetized measures for welfare evaluation. The aim of this study was to assess the welfare status of 16 Sardinian dairy cattle farms, based on the developed Animal Welfare and Biosecurity Evaluation checklist (AWB-EF) and the corresponding hematological, biochemical, and electrophoretic profiles of these animals.
View Article and Find Full Text PDFMeasurement of anti-natalizumab antibodies by homogeneous mobility shift assay.
Pathology
April 2020
Haematology Research Group, Christchurch Hospital, New Zealand.
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