1. Peptide T and four other aminoacyl derivatives of this octapeptide were synthesized on solid phase support using the Boc and Fmoc procedures. 2. The octapeptides were modified by chloroacetylation and radiolabelled by halogen exchange with 125I. 3. Purified and crude extracts of lymphocytes were used to determine the binding of the octapeptides at different concentrations.
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http://dx.doi.org/10.1016/0020-711x(92)90267-5 | DOI Listing |
Int J Mol Sci
January 2025
Biotechnology Department, Sirius University of Science and Technology, 354349 Sirius, Russia.
In addition to the 20 canonical amino acids encoded in the genetic code, there are two non-canonical ones: selenocysteine and pyrrolysine. The discovery of pyrrolysine synthetases (PylRSs) was a key event in the field of genetic code expansion research. The importance of this discovery is mainly due to the fact that the translation systems involving PylRS, pyrrolysine tRNA (tRNA) and pyrrolysine are orthogonal to the endogenous translation systems of organisms that do not use this amino acid in protein synthesis.
View Article and Find Full Text PDFMol Cancer
January 2025
Department of Cell Biology, Physiology, and Immunology, University of Córdoba, CIBER Pathophysiology of Obesity and Nutrition (CIBERobn), Córdoba, 14004, Spain.
Background: Hepatocellular carcinoma (HCC) genetic/transcriptomic signatures have been widely described. However, its proteomic characterization is incomplete. We performed non-targeted quantitative proteomics of HCC samples and explored its clinical, functional, and molecular consequences.
View Article and Find Full Text PDFArch Biochem Biophys
January 2025
Department of Biotechnology, Maharaja Sriram Chandra Bhanjadeo University, (Erstwhile: North Orissa University), Baripada, Odisha, 757003, India. Electronic address:
Our previous study revealed that lipid flip-flop inducing phytochemicals from Gymnema sylvestre increase membrane permeability of antimicrobials in S. aureus. However, their lipid flipping and membrane permeabilizing effect on methicillin resistant S.
View Article and Find Full Text PDFAm J Physiol Lung Cell Mol Physiol
February 2025
Department of Medicine, Section of Pulmonary and Critical Care Medicine, The University of Chicago, Chicago, Illinois, United States.
Idiopathic pulmonary fibrosis is a fatal disease characterized by the transforming growth factor (TGF-β)-dependent activation of lung fibroblasts, leading to excessive deposition of collagen proteins and progressive replacement of healthy lungs with scar tissue. We and others have shown that TGF-β-mediated activation of the mechanistic target of rapamycin complex 1 (mTORC1) and downstream upregulation of activating transcription factor 4 (ATF4) promotes metabolic reprogramming in lung fibroblasts characterized by upregulation of the de novo synthesis of glycine, the most abundant amino acid found in collagen protein. Whether mTOR and ATF4 regulate other metabolic pathways in lung fibroblasts has not been explored.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, 24341, South Korea; Future F Biotech Co., Ltd, Chuncheon 24341, South Korea. Electronic address:
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