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Cloning and functional characterization of sesquiterpene synthase genes from Inonotus obliquus using a Saccharomyces cerevisiae expression system.
World J Microbiol Biotechnol
January 2025
Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou, 221116, China.
Inonotus obliquus (Chaga mushroom) is a large medicinal and edible fungus that contains a wealth of bioactive terpenoids. However, the detection of certain low-abundance sesquiterpenoids remains a challenge due to limitations in extraction and analytical techniques. Furthermore, the synthase genes responsible for the biosynthesis of the identified terpenoids have not yet been clearly elucidated.
View Article and Find Full Text PDF: identification and characterization of plant-conserved microRNA in Rafflesiaceae.
Open Life Sci
January 2025
Department of Biology, Pace University, One Pace Plaza, 3rd Floor, New York, 10038, NY, United States of America.
Rafflesiaceae is a family of endangered plants whose members are solely parasitic to the tropical grape vine (Vitaceae). Currently, the genetics of their crosstalk with the host remains unexplored. In this study, we use homology-based approaches to characterize micro-RNAs (miRNAs) expressed by and from published omics data.
View Article and Find Full Text PDFMechanisms and new advances in the efficacy of plant active ingredients in tendon-bone healing.
J Orthop Surg Res
January 2025
The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, No.41 Linyin Road, Baotou, Inner Mongolia, 014010, China.
The tendon-bone interface, known as the tenosynovial union or attachment, can be easily damaged by excessive exercise or trauma. Tendon-bone healing is a significant research topic in orthopedics, encompassing various aspects of sports injuries and postoperative recovery. Surgery is the most common treatment; however, it has limited efficacy in promoting tendon-bone healing and carries a risk of postoperative recurrence, necessitating the search for more effective treatments.
View Article and Find Full Text PDFEcdysteroids in Native Trees and Callus Cultures of the Genus Vitex L. of the Flora of Russia and Vietnam.
Phytochem Anal
January 2025
Institute of Biology, Federal Research Centre Komi Science Center, Ural Branch of the Russian Academy of Sciences, Syktyvkar, Russia.
Introduction: Vitex L. is a large genus of tropical and subtropical trees used in medicine of many nations. Some species are used in gynecology due to flavonoids, iridoids, and diterpenes.
View Article and Find Full Text PDFDestined for destruction: The role of methionine aminopeptidases and plant cysteine oxidases in N-degron formation.
Plant Physiol
December 2024
Department of Biology, University of Oxford, South Parks Road, Oxford OX1 3RB, UK.
The cysteine/arginine (Cys/Arg) branch of the N-degron pathway controls the stability of certain proteins with methionine (Met)-Cys N-termini, initiated by Met cleavage and Cys oxidation. In seeding plants, target proteins include the Group VII Ethylene Response Factors, which initiate adaptive responses to low oxygen (hypoxic) stress, as well as Vernalization 2 (VRN2) and Little Zipper 2 (ZPR2), which are involved in responses to endogenous developmental hypoxia. It is essential that these target proteins are only degraded by the N-degron pathway under the appropriate physiological conditions.
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