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A modified fast micro method in agarose for isotype, allotype, light chain and idiotype-specific analysis of antibody clonotypes to bacterial virulence antigens.
Scand J Clin Lab Invest
December 1998
Department of Clinical Immunology, University of Göteborg, Sweden.
A modified fast micro method for spectrotypic/clonotypic analysis of human IgG1-4 antibodies against bacterial virulence antigens of polysaccharide or protein nature is described. Serum samples of as small volumes as 0.5 microliter were isoelectrically focused in micro agarose gels made in plexiglass matrices and blotted using immunoaffinity-mediated capillary blotting onto nitrocellulose membranes previously coated with antigen.
View Article and Find Full Text PDFObjective: To determine if Staphylococcus aureus and Staphylococcus epidermidis, etiologic bacterial agents to late prosthetic joint infections (LPJI), are more prevalent in the oral flora of older individuals with rheumatoid arthritis (RA) than in an age and gender-matched nonarthritic control population (NA).
Design: Cultures were obtained from the nares, oropharynx, saliva, tongue, and gingival crevice, and the results were compared between older patients with RA and controls.
Setting: University of Michigan Medical Center, Ann Arbor, VA Medical Center, and University of Michigan School of Dentistry.
Comparative evaluation of three antifungal susceptibility test methods for Candida albicans isolates and correlation with response to fluconazole therapy.
J Clin Microbiol
December 1996
Abteilung Innere Medizin and Poliklinik, Virchow-Klinikum, Humboldt-Universität, Berlin, Germany.
In vitro susceptibilities were determined for 56 Candida albicans isolates obtained from the oral cavities of 41 patients with human immunodeficiency virus infection. The agents tested included fluconazole, itraconazole, ketoconazole, flucytosine, and amphotericin B. MICs were determined by the broth microdilution technique following National Committee for Clinical Laboratory Standards document M27-P (M27-P micro), a broth microdilution technique using high-resolution medium (HR micro), and the Etest with solidified yeast-nitrogen base agar.
View Article and Find Full Text PDFThe identification of antigenic determinants by a coupled inhibition-agar diffusion method.
J Immunol Methods
December 1984
The determination of the chemical nature of immunodeterminant groups of carbohydrate antigens has been achieved by a micro method coupling inhibition and double diffusion in agar. This method has been tested with antigens which react with anti-lactose, anti-galactose, anti-N-acetyl-glucosamine and antirhamnose antibodies. The analysis can be performed with as little as 10 micrograms of inhibitor, 0.
View Article and Find Full Text PDFOptimal conditions were established for a micro method for the production of colonies of B lymphocytes from mouse spleen cells cultured in agar in glass capillaries, in the presence of bacterial lipopolysaccharide (LPS). Besides LPS the cultures require 5 X 10(-5) M mercaptoethanol and 20% horse serum for optimal colony growth. Foetal calf serum and heat-inactivated horse or foetal calf serum were found to be inferior.
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