Actin is one of the most abundant proteins in the motile intestinal protozoan parasite E. histolytica. A number of actin gene copies have been detected. The cDNA and genomic sequences of two of the actin genes have been independently reported (1,2). Almost complete homology was detected between the coding regions of the two genes; however, significant differences were observed in the sequences of their 5' untranslated regions. Using the coding region of actin as the focal point, we performed a chromosome walk to identify the neighboring genes and the intergenic regulatory domains. A genomic library containing large fragments of DNA was screened with the coding and non-coding regions of the actin gene. An insert of 8.5 kb reacted on Northern blots with actin and two additional transcripts. The large (approximately 2.5 kb) transcript has not yet been identified, but the smaller one (600 bp), was shown to encode for the ribosomal protein L21. Both the cDNA and genomic sequences of this gene were determined. The RP-L21 gene was found to be physically connected to the actin gene by a 2.1 kb intergenic stretch. The actin gene on this DNA fragment contained a 5' untranslated region that was identical to the sequence described by Edman et al. The actin gene isolated by Huber et al. was located by PFGE on another chromosomal band that did not contain the RP-L21 gene.
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Acta Parasitol
January 2025
Parasitology Department, Theodor Bilharz Research Institute, Giza, 12411, Egypt.
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Department of Pediatrics, Erciyes University, Faculty of Medicine, Kayseri, Turkey.
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Department of Biochemistry, Dongguk University College of Medicine, 123 Dongdae-ro, Gyeongju 38066, Republic of Korea.
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January 2025
Department of Cellular Pathology, Institute for Developmental Research, Aichi Developmental Disability Center, Kasugai 480-0392, Aichi, Japan.
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January 2025
Department of Orthopedics, Quanzhou First Hospital Affiliated to Fujian Medical University, 362000 Quanzhou, Fujian, China.
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