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Genomic microsatellite characterization and development of polymorphic microsatellites in Eospalax baileyi.
Sci Rep
January 2025
Key Laboratory of Grassland Ecosystem (Ministry of Education), Pratacultural College, Gansu Agricultural University, Lanzhou, 730070, China.
Microsatellite markers are cost-effective, rapid, efficient, and show great advantages in in large-sample kinship analysis and population structure studies. However, microsatellite loci are seriously underdeveloped in non-model organisms. The plateau zokor (Eospalax baileyi) is a key species living underground in the Tibetan Plateau, the effective management of which has long been challenging.
View Article and Find Full Text PDFIFIT proteins (interferon-induced proteins with tetratricopeptide repeats) are key components of the innate immune response that bind to viral and cellular RNA targets to inhibit viral translation and replication. The RNA target recognition is guided by molecular patterns, particularly at the RNA 5' ends. IFIT1 preferably binds RNAs modified with the 7-methylguanosine (m7G) cap-0 structure, while RNAs with cap-1 structure are recognized with lower affinity.
View Article and Find Full Text PDFmiR158a negatively regulates plant resistance to Phytophthora parasitica by repressing AtTN7 that requires EDS1-PAD4-ADR1 complex in Arabidopsis thaliana.
Plant J
December 2024
State Key Laboratory of Crop Stress Resistance and High-Efficiency Production, and College of Agronomy, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Small RNAs are involved in diverse cellular processes, including plant immunity to pathogens. Here, we report that miR158a negatively regulates plant immunity to the oomycete pathogen Phytophthora parasitica in Arabidopsis thaliana. By performing real-time quantitative PCR, transient expression, and RNA ligase-mediated 5' rapid amplification of cDNA ends assays, we demonstrate that miR158a downregulates AtTN7 expression by cleaving its 3'-untranslated region.
View Article and Find Full Text PDFGenome-wide identification of SSR markers from coding regions for endangered Argania spinosa L. skeels and construction of SSR database: AsSSRdb.
Database (Oxford)
November 2024
Microbiology and Molecular Biology Team, Center of Plant and Microbial Biotechnologies, Biodiversity and Environment, Faculty of Sciences, Mohammed V University, 4 Avenue Ibn Batouta, B.P. 1014, Rabat 10000, Morocco.
Article Synopsis
- Microsatellites, or simple sequence repeats (SSRs), are important genetic markers in plants, but a thorough identification in the Argania spinosa L. genome has not been done until now.
- This study identified 5351 SSRs from 66,280 coding sequences (CDSs) in A. spinosa, with tri-nucleotide motifs being the most common.
- An online database (AsSSRdb) was created to share these SSRs, providing resources for DNA fingerprinting and genetic studies in argan and similar species.
Development of Simple Sequence Repeat of (Coleoptera: Cerambycidae) Based on Restriction Site-Associated DNA Sequencing.
Biology (Basel)
October 2024
Guangxi Colleges and Universities Key Laboratory for Cultivation and Utilization of Subtropical Forest Plantation, Guangxi Key Laboratory of Forest Ecology and Conservation, College of Forestry, Guangxi University, Nanning 530004, China.
, a pest posing a serious threat to coniferous species, such as , has had devastating effects on pine forests due to its association with . The creation of unique simple sequence repeat (SSR) primers for is crucial, as there has been little study of the species' phylogeography. The aim of this study was to identify and create polymorphic SSR primers by sequencing samples of obtained from three different sampling points using the restriction site-associated DNA sequencing (Red-seq) approach.
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