Viral suspensions of herpetic virus were inoculated on different cell cultures: human diploid cells (HDC) and monkey kidney cells--primary culture and cell line--for adapting the virus on these cells and obtaining several high constant titers. The human diploid cells and the monkey kidney cell line have proved to be the most suitable cells for the virus growth; thus an optimum development of the virus with a high constant titer was obtained starting with the 4th passage (10(5.7)-10(6)CPD/50/ml). The optimum conditions provided for the herpetic virus culture have allowed to obtain a herpetic virus stock used as control preparation for testing the efficacy of the "OFTALMOHERPIN" biological preparation for current use in the prophylaxis and therapy of ocular herpes.
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